摘要
为解析花生耐旱性的调控基础,本研究通过对10个不同的花生材料苗期进行干旱-复水实验,筛选抗感材料进行转录组分析,探讨了干旱条件下不同花生材料抵御干旱胁迫的分子机制。研究结果显示,来源于非洲的花生材料Waliyar Tiga耐旱性最强,其次是k Q044抗青、中花16和早花生,干旱敏感的材料为狮头企、山花13、ICGV86745以及丰花2号;耐旱及干旱敏感材料的根冠比存在显著差异,耐旱材料的根冠比平均值为35.0%,干旱敏感材料的根冠比平均值为15.26%。早花生和中花16的根冠比最大。转录组结果表明抗感材料的差异表达基因主要富集在氧化磷酸化、光合作用和植物代谢途径;通过差异基因富集分析发现,耐旱材料在干旱条件下生长素应答途径基因的表达明显弱于敏感材料。生理和转录组的结果表明耐旱材料利用发达的根系系统、能量代谢的提升、次生代谢的加强和生长的抑制四个方面共同应对干旱胁迫。抗旱材料中花16和Waliyar Tiga在干旱条件下均具有较强的光合作用和氧化磷酸化的能力,中花16的根冠比显著大于Waliyar Tiga,但其耐旱性不及Waliyar Tiga,推测可能源于其较大的叶面积导致更多的叶面水分散失,从而使其耐旱能力低于Waliyar Tiga。
In order to elucidate the regulating basis of drought tolerance in peanut,the experiments of drought and re-water after drought were carried out on 10 different peanut accessions to select drought tolerant and sensitive lines,combined with the transcriptome to analysis the regulation mechanism of drought resistance of different peanut lines. Waliyar Tiga,which was collected from Africa,showed the best drought tolerance,followed by k Q044 kangqing,Zhonghua 16 and Zaohuasheng. The physiological results showed that there were significant differences in root/shoot ratio between drought-tolerant and drought-sensitive peanuts. Transcriptome analysis demonstrated that differential expressed genes between drought tolerant and susceptible peanut lines were obviously enriched in oxidative phosphorylation,photosynthesis and secondary metabolites pathways; the genes expression of auxin signal pathway in drought-tolerant peanuts lines were significantly lower than that in the susceptible ones.The physiological and transcriptome data demonstrated that enlarged root system,enhanced energetic,secondary metabolism and the inhibition of growth were the four aspects contributing to drought stress tolerance in peanut.Both Zhonghua 16 and Waliyar Tiga have vigorous root and energetic metabolism,and the root shoot ratio was even larger in Zhonghua 16 than Waliyar Tiga,the reason for the conflict could be caused by the larger leaf area which resulted greater water loss. This study provided a theoretical basis for drought-tolerant peanuts breeding,and provided a way to the further study drought tolerance regulation mechanism of peanut.
作者
万丽云
苏威
李蓓
雷永
晏立英
康彦平
淮东欣
陈玉宁
姜慧芳
廖伯寿
WAN Li-yun;SU Wei;LI Bei;LEI Yong;YAN Li-ying;KANG Yan-ping;HUAN Dong-xin;CHEN Yu-ning;JIANG Hui-fang;LIAO Bo-shou(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetics Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,Chin)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2018年第3期335-343,共9页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31461143022)
国家花生产业技术体系(CARS-14)
关键词
花生
耐旱
转录组
根系
peanut
drought tolerance
transcriptome
root system
作者简介
万丽云,助理研究员,博士,主要从事植物逆境分子生物学研究,E-mail：wanliyun＠caas.cn;苏威,主要从事作物遗传育种研究,E-mail：sujuzi＠139.com;通讯作者：廖伯寿,主要从事花生遗传育种及病害研究,E-mail：lboshou＠hotmail.com
