摘要
运用实时荧光定量PCR技术探讨了不同供氮条件下强化感与弱化感水稻苯丙烷代谢途径中9个关键酶基因的表达差异。结果表明,与正常氮素供应相比,低氮胁迫引起强化感水稻‘P1312777’中与酚类代谢途径相关的9个关键酶基因表达量均上调,表达量增幅在1.9～5.4倍之间,且以PAL基因上调倍数最大。而弱化感水稻‘Lemont’则相反,只有2个基因(苯丙氨酸裂解酶基因和肉桂酰CoA基因)表达上调,但上调倍数分别是强化感水稻对应的基因的22%和74%,其余的7个基因表达均下调,降幅在29%～72%之间,表明低氮胁迫诱发的水稻化感抑草能力增强与其体内酚类物质合成代谢增强有关。
Differential expression of the key genes controlling phenolic metabolism in allelopathic and non-allelopathic rice accessions was investigated under two nitrogen supply levels （lower and normal） using fluorescence quantitative-poly- merase chain reaction （FQ-PCR） （Figs. 2, 3）. The results indicated that 9 key enzyme genes con- cerned were mediated by lower nitrogen level （Table 2）. All of the nine genes （Table 1, Fig.4）, were up-regulated by 1.9-5.4 times of the relative gene expression amounts in allelopathic rice accession, ＇PI312777＇ under the lower nitrogen condition compared with their controls, of which PAL gene showed the highest relative gene expres- sion amount with 5.4 times of the relative gene expressions compared with the control, while in non-allelopathic rice Lemont, seven genes were down-regulated by 29%-72% under lower nitro-gen supplies compared with their controls and only two genes, i.e., phenylalanine ammonia-lyase and cinnamoyl-CoA genes were up-regulated, which however were a decrease of 22% and 74% over those in allelopathic rice accession （Table 2）. These findings strongly suggest that the increase of al- lelopathic potential induced by 1/4 nutrient stress was responsible for enhanced phenolic compound synthesis metabolism.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2007年第5期387-394,共8页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金(Nos.30471028
30070068)资助。~~
关键词
水稻
苯丙烷代谢途径
实时荧光定量聚合酶
链式反应
氮素
Oryza sativa
phenylalanine metabolism
real-timefluorescence quantitative-polymerase chain reaction （FQ-PCR）
nitrogen elements
作者简介
通讯作者（E-mail：wenxiongl81@163．com；Tel：0591-83769440）。
