摘要
NAC转录因子在植物应答非生物胁迫中起重要作用。利用生物信息学分析推测花生栽培种转录因子基因Ah NAC4（登录号为HM776131.1）属于抗旱相关转录因子基因,对比栽培品种山花11号Ah NAC4的2条c DNA序列（Shr NAC4-a和Shr NAC4-b）及其相应的DNA序列（Sh NAC4-a和Sh NAC4-b）表明,Ah NAC4全长为1244 bp,编码区长度为1050 bp,含有2个内含子,分别位于182~279 bp和547~642 bp处,编码蛋白包含349个氨基酸。从抗旱性不同的32个栽培品种分离得到4类Ah NAC4,分别命名为Ah NAC4-a1、Ah NAC4-a2、Ah NAC4-b1和Ah NAC4-b2,缩写为a1、a2、b1和b2。a1和a2为等位基因,二者在717 bp处存在1个碱基差异,引起第174位氨基酸的改变,b1和b2为等位基因,二者存在14个SNP位点,其中717 bp和924 bp处碱基的差异引起第174位和第244位氨基酸的改变。供试品种中基因型为a1a1b1b1、a1a1b2b2、a2a2b1b1、a2a2b2b2的品种数分别为10、5、15和2。从19个野生种中分离得到11类NAC4的DNA序列（Aw1NAC4–Aw11NAC4）,Aw1NAC4与栽培种b1、b2的核苷酸序列同源性最高,Aw2NAC4与栽培种a1、a2核苷酸序列同源性最高。推测栽培种a1基因编码蛋白对花生抵御干旱起关键作用,a1和b1基因编码蛋白的功能与野生种更接近。
NAC transcription factors play an important role in response to abiotic stress of plant. In this paper, bioinformatic analysis indicated that transcription factor gene Ah NAC4（accession number HM776131.1） can response to drought signal. The comparison of c DNAs（Shr NAC4-a and Shr NAC4-b） and genomic DNAs（Sh NAC4-a and Sh NAC4-b） from Shanhua 11 showed that Ah NAC4 has a full length of 1244 bp and an ORF of 1050 bp, containing two introns at 182–279 bp and 547–642 bp, and encoding 349 amino acids. Four kinds of Ah NAC4 genes, named as Ah NAC4-a1, Ah NAC4-a2, Ah NAC4-b1, and Ah NAC4-b2（abbreviations： a1, a2, b1, and b2）, were cloned from 32 cultivars with different drought resistances. Among them a1 and a2 were alleles, with only one locus different between the two genes, leading to the difference of corresponding amino acids at site 174; b1 and b2 were alleles, with 14 SNPs, of which two SNPs led to differences of the corresponding amino acids at sites 174 and 244. There were four genotypes of Ah NAC4 in the 32 cultivars, containing 10 a1a1b1b1, 5 a1a1b2b2, 15 a2a2b1b1, and 2 a2a2b2b2. Meanwhile, 11 NAC4 genes（Aw1NAC4–Aw11NAC4） were isolated from 19 wild peanut accessions in Arachis. Aw2NAC4 had the highest homology of nucleotide sequence with a1 or a2, and b1 or b2 had the highest homology of nucleotide sequence with Aw1NAC4. It is speculated that the protein encoding a1 plays a key role in responding drought stress, and the function of the proteins encoding a1 and b1 genes is closer with that of wild species.
出处
《作物学报》
CAS
CSCD
北大核心
2015年第1期31-41,共11页
Acta Agronomica Sinica
基金
山东省花生良种产业化工程项目
山东省现代农业产业技术体系花生创新团队建设项目(SDAIT-05-011-02)
国家现代农业产业技术体系建设专项(CARS-14)
国家"十二五"科技支撑计划项目(2011BAD35B04)
国家自然科学基金项目(31271757)资助
关键词
花生
转录因子
NAC
等位变异
Peanut
Transcription factor
NAC
Allelic variation
作者简介
刘风珍，E-mail：liufz@sdau．edu．cn，Tel：0538-8241540
