摘要
为探讨盾叶薯蓣在低磷胁迫下的生理变化、甾体皂苷类成分代谢及基因表达的响应特征,本研究选取河南南阳产盾叶薯蓣进行模拟低磷胁迫实验,在不同时期对根际基质中的磷含量(全磷、速效磷、磷酸铝盐、磷酸铁盐、磷酸钙盐)和土壤酸性磷酸酶(soil acid phosphatase,S-ACP)活性、植株根系发育特征(总根长、总投影面积、总表面积),各部位过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)活性及甾体皂苷类成分含量等指标进行分析,确定盾叶薯蓣响应低磷胁迫的关键时期,并利用RNA-Seq测序对关键时期盾叶薯蓣根茎、叶片、地上茎3个部位中的基因表达特征进行分析。研究发现低磷胁迫处理后盾叶薯蓣根际基质中易吸收态磷含量显著降低,其抗氧化酶(POD、SOD)与酸性磷酸酶活性均显著升高,根系发育受阻;低磷胁迫可明显影响盾叶薯蓣中甾体皂苷的合成与积累,且不同部位响应特征不同;胁迫初期为盾叶薯蓣响应低磷胁迫的关键时期;响应低磷胁迫关键时期的盾叶薯蓣基因表达存在明显的组织特异性,对三个处理组不同部位基因表达量与代谢通路进行分析,分别从盾叶薯蓣根茎、叶片、地上茎3个部位中挖掘到239、211、237个差异基因,涉及萜类骨架、有机酸、肌醇的生物合成等多个代谢通路。上述研究结果说明盾叶薯蓣通过改变基因表达水平对表型性状和生理代谢过程进行调节来响应低磷胁迫,本研究为研究盾叶薯蓣响应低磷胁迫的分子机制提供理论依据。
To investigate the physiological changes,steroidal saponins metabolism and gene expression of D.zingiberensis under low phosphorus stress,D.zingiberensis from Nanyang,Henan province was selected to simulate low phosphorus stress.The phosphorus content(total phosphorus,available phosphorus,aluminophosphate,phosphoric acid iron salt,calcium phosphate)and soil acid phosphatase(S-ACP)activities in rhizosphere matrix,root development characteristics(total root length,total projected area,total surface area),peroxidase,superoxide dismutase activities and steroid saponins contents in each tissue were analyzed at different periods.The key period of D.zingiberensis response to low phosphorus stress was determined,and RNA-seq sequencing was used to analyze the gene expression characteristics in roots,leaves and stems of D.zingiberensis.It was found that the content of absorbable phosphorus in rhizosphere matrix of D.zingiberensis was significantly decreased,the activities of antioxidant enzymes(POD,SOD)and soil acid phosphatase(S-ACP)were significantly increased under low phosphorus stress,and root development was inhibited;The synthesis and accumulation of steroidal saponins in D.zingiberensis were obviously affected by low phosphorus stress,and the response characteristics of different parts were different;The early stage of stress is the critical period for D.zingiberensis to respond to the low phosphorus stress;The gene expression of D.zingiberensis in the critical period of low phosphorus stress showed obvious tissue specificity.The gene expression levels and metabolic pathways in different parts of the three treatment groups were analyzed,and 239,211 and 237 differential genes were found from the root,leaf and stem of D.zingiberensis,respectively.It involves many metabolic pathways such as terpenoid skeleton,organic acid and inositol biosynthesis.et al.The above research results indicate that D.zingiberensis responded to the low phosphorus stress by regulating phenotypic traits and physiological metabolic process through changing gene expression level,which provided theoretical basis for studying the molecular mechanism of D.zingiberensis response to the low phosphorus stress.
作者
王庆婷
耿晓桐
李雅静
张娟
刘庆普
龚海燕
雷敬卫
谢彩侠
WANG Qing-ting;GENG Xiao-tong;LI Ya-jing;ZHANG Juan;LIU Qing-pu;GONG Hai-yan;LEI Jing-wei;XIE Cai-xia(Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine,Henan University of Traditional Chinese Medicine,Zhengzhou 4500462,China;Xinyang Agriculture and Forestry College,Xinyang 464000,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第9期1569-1582,共14页
Natural Product Research and Development
基金
河南省高等学校重点科研项目(20A360016)。
关键词
盾叶薯蓣
低磷胁迫
磷形态
土壤酸性磷酸酶
抗氧化酶
甾体皂苷
基因表达
Dioscorea zingiberensis C.H.Wright
low phosphorus stress
phosphorus form
soil acid phosphatase
antioxidant enzymes
steroidal saponins
gene expression
作者简介
通信作者:谢彩侠,Tel:86-013673651577,E-mail:nanyangxcx@126.com。
