摘要
干旱胁迫下氮素施用对植物生长发育具有重要的影响。为明确氮素提高花生抗旱性的生理和转录调控机制,本研究对施氮、干旱及旱氮同存处理下的花生生理指标和根系转录组进行了测定。结果表明,旱氮同存处理提高了干旱胁迫下花生生物量和叶片相对含水量。施用氮肥增加了干旱胁迫下花生根系的总酚和类黄酮含量,提高其过氧化物酶(POD)和过氧化氢酶(CAT)活性,降低其丙二醛(MDA)含量,提高花生抗旱性。转录组分析表明,施用氮肥产生5396个差异表达基因,这些基因主要参与谷胱甘肽代谢、氮代谢和碳代谢相关过程及应激和防御反应。干旱处理和旱氮同存处理下,次生代谢物生物合成、运输和分解代谢及碳水化合物运输和代谢这两类功能差异表达基因富集。旱氮同存处理下酚类代谢物质相关的3种途径中有51个差异基因上调表达,207个基因下调表达。由此表明,施用氮肥通过调控花生次生产物代谢、碳水化合物代谢等途径提高干旱胁迫下花生植株的抗氧化能力,从而提高花生的抗旱性。
Nitrogen application has an important effect on plant growth and development under drought stress.The aim of this study is to clarify the physiological and transcriptional regulation mechanism of nitrogen for improving drought resistance in peanut.The physiological indexes and root transcriptome of peanut under nitrogen application,drought stress and drought and nitrogen application simultaneously exist treatments were determined.The results showed that the drought and nitrogen application simultaneously exist treatment increased peanut biomass and the relative water content of leaves under drought stress.The content of total phenols and flavonoids in peanut roots were increased by nitrogen application under drought stress.Meanwhile,the activities of POD and CAT were increased,the content of MDA was decreased,which improved the drought resistance of peanut.Transcriptome analysis showed that the relative expression of 5396 genes changed due to nitrogen application.These genes were mainly involved in glutathione metabolism,nitrogen metabolism,and carbon metabolism,as well as stress and defense responses.Under drought stress,the drought and nitrogen application simultaneously exist treatments,the differentially expressed genes of secondary metabolite biosynthesis,transportation and catabolism and carbohydrate transport and metabolism were enriched.Among the three pathways related to phenolic metabolites,51 differential genes were up-regulated and 207 genes were down-regulated under the drought and nitrogen application simultaneously exist treatment.In conclusion,the application of nitrogen fertilizer could enhance the antioxidant capacity of peanut plants under drought stress through regulating secondary metabolites and carbohydrate metabolism,and thus improve the drought resistance of peanut.
作者
丁红
张智猛
徐扬
张冠初
郭庆
秦斐斐
戴良香
DING Hong;ZHANG Zhi-Meng;XU Yang;ZHANG Guan-Chu;GUO Qing;QIN Fei-Fei;DAI Liang-Xiang(Shandong Peanut Research Institute,Qingdao 266100,Shandong,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第1期225-238,共14页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31971854,31971856)
山东省花生产业技术体系(SDAIT-04-06)
山东省重大科技创新工程项目(2019JZZY010702)资助。
关键词
花生
干旱
氮素
抗氧化系统
转录组
peanut
drought stress
nitrogen
antioxidant system
transcriptome analysis
作者简介
通信作者:戴良香,E-mail:liangxiangd@163.com;第一作者:丁红,联系方式,E-mail:dingpeanut@163.com。
