摘要
目的：研究六君子汤乙酸乙酯提取物对食管癌Eca109细胞增殖的影响.方法：以104个/孔的细胞密度接种96孔板中,培养24 h后,各组分别加入不同浓度六君子汤提取物的培养液100 μL,质量浓度为10,20,40,60,80,100 mg·L^-1,另设空白孔,每组设8个复孔,采用MTT比色法检测六君子汤提取物对食管癌Eca109细胞株生长抑制作用,采用流式细胞仪检测药物作用后细胞凋亡情况,激光共聚焦显微镜观察药物作用后细胞形态变化.结果：在t0～100 mg·L^-1内,不同浓度六君子汤提取物能有效抑制Eca109细胞增殖（P＜0.05）.21.06,49.65,67.47 mg·L-1的六君子汤提取物作用Eca109细胞48 h,细胞凋亡率明显增加,与空白组相比,差异有统计学意义（P＜0.05）;并且在激光共聚焦显微镜下观察到凋亡小体,与空白组相比,早期凋亡和晚期凋亡的细胞数量明显增多.结论：六君子汤提取物能显著抑制食管癌Eca109细胞增殖,诱导细胞凋亡,其作用机制有待进一步研究.
Objective ： To study the effect and mechanism of Liujunzi Tang extract on the proliferation of human esophageal carcinoma cell. Method： Esophageal carcinom Ecal09 ceils were grown in 96-well plates and treated with different concentrations of Liujunzi Tang extract （10, 20, 40, 60, 80, 100 mg·L^-1）. There were 8 wells for each concentrations of Liujunzi Tang extract groups and for the normal group. Inhibitive effect of Liujunzi Tang extract on proliferation was detected by MTT assay, cell apoptosis was detected by flow cytometer and cellular morphology was observated by laser scanning confocal microscopy. Result： Liujunzi Tang extract produced a dose- dependent inhibition on the proliferation of Ecal09 cells within the concentration of 10-100 mg·L^-1 （p 〈 0.05）. Compared to the normal group, Liujunzi Tang extract could significantly increase the apoptosis rate of Ecal09 cells after being cultered for 48 h at the concentration of 21.06, 49.65, 67.47 mg·L^-1（P 〈0.05） , and the numbers of the apoptotic cells in the early or late stages were increased obviously. Conclusion： Liujunzi Tang extract could significantly inhibit the proliferation of Ecal09 cells by inducing cell apoptosis. Its exact mechanism will be further investigated.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第1期163-166,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173177)
河南中医学院苗圃工程项目(MP2012-02)
作者简介
尹素改，硕士，讲师，从事肿瘤病机与防治研究，Tel：0371-65680206，E-mail：yinsugai@163．com
[通讯作者]陈玉龙，博士，教授，从事肿瘤病机与防治研究，Tel：0371-65680206，E-mail：eyl72621@163．com
