摘要
目的:从中药蜈蚣体内提取多糖组分,并对其部分性质进行分析。方法:从中药蜈蚣体内提取粗多糖,经DEAE-52离子交换柱层析分离获得3个糖组分;凝胶过滤法测组分Ⅰ的分子量;MTT法检测多糖组分Ⅰ对两种肿瘤细胞的抑制作用。结果:组分Ⅰ的分子量为33.1kD;该多糖组分对Hela细胞的抑制作用明显,当糖浓度为3.13μg/mL时,抑制率达60.8%,但对Eca109细胞无明显抑制作用。结论:蜈蚣多糖组分Ⅰ对不同的肿瘤细胞具有特异性。
Objective： To obtain polysaccharide from Scolopendra subspinipes mutilans and study its partial properties. Methods： A raw polysaccharide was isolated from Chinese medicine Scolopendra subspinipes mutilans, and three groups were isolated by DEAE- 52. The thin layer chromatography and gel filtration chromatography were used to detect the main monosaccharide composition and mo- lecular weight of the group Ⅰ.The inhibitory effect of the group Ⅰ on tumor ceils was detected by MTT assay. Results： The molecular weight of the group Ⅰ was 33. 1 kD. The inhibitory effect of the group Ⅰon Hela cells was obvious, as its inhibitive rate was 60. 8% on Hela ceils when the polysaccharide＇s concentration was 3.13 μg/mL, but it had no effect on the Eca 109 ceils. Conclusion： The group I has specific effects on different tumor cells.
出处
《中药材》
CAS
CSCD
北大核心
2009年第6期846-848,共3页
Journal of Chinese Medicinal Materials
关键词
蜈蚣
多糖
分离纯化
肿瘤细胞
Scolopendra subspinipes mutilans
Polysaccharide
Isolation and purification
Tumor cells
作者简介
李兴暖（1976-），女，博士，从事中药研究与开发；Tel：0754-8900530，E—mail：li_xingnuan@126．com。
