摘要
【目的】研究启膈散乙酸乙酯部位对食管癌Eca109细胞生长的影响及其作用机制。【方法】采用四甲基偶氮唑盐（MTT）法检测启膈散乙酸乙酯部位对食管癌Eca109细胞的生长抑制作用,用流式细胞仪检测细胞凋亡情况,Western blot法检测STAT3、Bcl-2、Caspase9蛋白的表达量。【结果】在10~100μg/m L范围内,不同浓度启膈散乙酸乙酯部位能有效抑制Eca109细胞增殖（P〈0.05）。用1.47、33.26、76.52μg/m L的启膈散乙酸乙酯部位作用Eca109细胞48 h,细胞凋亡率显著增加,与对照组比较,差异均有统计学意义（P〈0.01）;Western blot检测结果显示：随着药物浓度的增加,STAT3和Bcl-2的表达量降低,Caspase9表达量增加。【结论】启膈散乙酸乙酯部位能显著抑制食管癌Eca109细胞增殖,并诱导细胞凋亡,其作用机制与抑制STAT3信号通路有关。
Objective To study the effect of Qige San ethyl acetate extract on the proliferation of human esophageal carcinoma Eca109 cells and to explore its possible mechanism. Methods Inhibitory effects of Qige San ethyl acetate extract on the proliferation of esophageal carcinoma Eca109 cells were detected by MTT assay, cell apoptosis was detected by flow cytometer, and the expression of protein STAT3, Bcl-2 and Caspase 9 was detected by Western blotting method. Results In the range of 10~100 μg/mL, Qige San ethyl acetate extract inhibited the proliferation of Eca109 cells effectively （P〈0.05） . Compared with the control group, Qige San ethyl acetate extract in the concentrations of 1.47, 33.26, 75.52 μg/mL significantly increased the apoptotic rate of Eca109 cells within 48h （ P〈0.01）. And Western blotting results showed that the ex pression levels of STAT3 and Bcl-2 were reduced, and Caspase 9 was increased with the increase of drug concentration. Conclusion Qige San ethyl acetate extract could significantly inhibit the proliferation of Eca109 cells and induce cell apoptosis, and its mechanism is probably associated with the inhibition of signal transducers and activators of transcription 3 （STAT3） signaling pathway.
出处
《广州中医药大学学报》
CAS
2015年第5期857-860,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81373535)
河南省高等学校重点科研项目(编号:15A310020)
作者简介
尹素改（1979-），女，讲师；E—mail：yinsugai@163．com
通讯作者：陈玉龙（1973-），男，教授；E-mail：cyl72621@163．com
