摘要
目的:研究通莲Ⅰ号方及其拆方在不同浓度下对食管癌Eca109细胞形态和生长周期的调节作用;探讨该方抑制食管癌细胞增殖的机制。方法:将Eca109细胞以1×106个/皿密度接种于Φ100 mm培养皿中,以10%小牛血清DMEM培养液培养,37℃,5%饱和湿度的CO2培养箱孵育,细胞贴壁24 h,分别加入浓度为25～3 200 mg.L-1倍比梯度的通莲Ⅰ号方(T)、清热解毒拆方(Q)、活血行气拆方(H)、滋阴养血拆方(Z)等4方提取液,孵育48 h后,MTT比色法检测细胞增殖变化、光镜观察细胞形态、流式细胞技术(FCM)测定细胞周期。结果:T,Q,H的IC50值分别为386,771,729 mg.L-1;与空白对照组(C)相比,T,Q,H 3方显著影响Eca109细胞周期变化:G1期细胞所占比率分别为69.43%,60.84%,61.90%。同时,T,Q,H组细胞的S期与C组比较,均有显著性差异(P<0.05),T方作用最强。而Z方对细胞的抑制作用较弱,与C组无显著性差异。结论:通莲I号方通过抑制细胞进入S增殖期从而干预食管癌Eca109细胞生长,各拆方具有协同作用,全方作用最优。
Objective： To investigate effect of Tonglian decoction （T） on proliferation of esophageal carcinoma cell line Ecal09 through determination of cell morphology and cell cycle, and to discuss inhibition mechanism of the prescription on esophageal carcinoma. Method： Human esophageal carcinoma cell line Ecal09 cultured in vitro were treated with T and its separated formulae, including clearing-heat-and-detoxification formula （Q） , activating-blood-and-promoting-Qi formula （ H ） and nourishing-Yin-and-blood formula （ Z ）. Cell proliferation was assayed by MTT, cells morphology was observed with microscope and cell cycle was measured with Flow Cyto Meter （FCM）. Result： Dose for 50 percent cells inhibition rates （ICs0） of T, Q and H was 386, 771, 729 mg · L^- 1, respectively. T, Q and H inhibit cell proliferation predominantly, rate of G1 phase of cell cycle reached to 69.43% , 60. 84% , 61.90% , respectively. Cells number in S phase treated with T, Q, and H had significant difference with controlled group （P 〈 0.05） , and T had the best effect. But Z has little inhibition power, having no difference with controlled group. Conclusion： T can be used to regulate Ecal09 carcinoma cell proliferation with inhibiting cells S phase. During whole process, 3 separated formulae cooperate with each other to insure effect of T.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期191-194,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
唐山市科学技术研究与发展计划课题(11150205A-7)
深圳市科技研发资金项目(ZYA201007060089A)
河北联合大学博士科研启动基金(BS09034),河北联合大学青年科学基金(QK032/2010)
作者简介
[通讯作者]贾永森，医学博士，讲师，从事肿瘤疾病中医方证基础研究，Tel：0315-3725103，E—mail：jiayongsen@126．com
