摘要
目的:观察比较不同健脾和胃方药的抗肿瘤作用及探讨其作用机制。方法:体外培养人食管癌细胞株Eca109和人肝癌细胞株HepG2,分离和培养人外周血单核细胞(PBMC),MTT和Alamar Blue法测定细胞生长。观察比较六君子汤(S)、四君子汤(F)、二陈汤(T)对Eca109和HepG2细胞的生长抑制作用及对PBMC激活作用,同时比较S、F、T激活的PBMC对Eca109和HepG2细胞生存的影响。结果:3方对Eca109和HepG2细胞的生长都有不同程度的抑制作用,随药物浓度增高而增强,具有明显的剂量依赖性。其中T作用最强,S次之。3方药对Eca109细胞的抑制作用强于对HepG2细胞的作用,但两者没有显著差异。3方对PBMC都有不同程度的激活作用,其中F最强,S次之。激活后的PBMC对Eca109和HepG2生长有明显抑制作用。结论:健脾和胃方药六君子汤、四君子汤、二陈汤可以通过直接抑制肿瘤细胞生长和通过免疫调节两个途径发挥其抗Eca109和HepG2细胞效应。二陈汤对肿瘤细胞生长抑制最强,四君子汤激活PBMC作用效果最好。
Objective：To observe and compare anti-tumor affects of different reinforcing spleen and stomach recipes and discuss its function mechanism.Methods：Human esophagus cancer cell line Eca 109 and liver cancer cell line HepG2 were in vitro cultured.Peripheral blood mononuclear cells（PBMC） were isoluted and cultured.MTT and Alamar Blue method were used to detect cell growth.Inhibitory effect of Liujunzi decoction（S）,Sijunzi decoction（F）,Erchen decoction（T） on Eca109 and the HepG2 cell＇s growth and activation effect on the PBMC were observed and compared.PBMC activation by S,F,T to Eca109 and the HepG2 cell survival influence were compared simultaneously.Results：The three recipes all can inhibit Eca109 and the HepG2 cell＇s growth to various degree and in a dose-dependent manner.T function is strongest,S the next best The inhibitory action of three recipes on Eca109 cell stronger than to HepG2 cell,but there was no statistical difference.The three recipes all have activation function to PBMC in various degree,F is the strongest,S the next best.PBMC activated has the obvious inhibitory action to Eca109 and the HepG2 growth.Conclusion：The reinforcing spleen and stomach recipes including Liujunzi decoction, Sijunzi decoction,Erchen decoction may play its anti-Eca109 and the HepG2 cell effect through two ways,one is to suppress the tumour cell growth directly,the other is to adjust the immunity.T suppresses the tumour cell growth strongly,F activates the PBMC function significantly.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第3期112-115,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
健脾和胃
四君子汤
六君子汤
二陈汤
抗肿瘤
reinforcing spleen and stomach
Liujunzi decoction
Sijunzi decoction
Erchen decoction
anti-tumor
作者简介
[通讯作者]陈玉龙，Tel：（0371）65680134：E-mail：cy172621@163．com
