摘要
本文研究了河南马氏钳蝎蝎毒（ＳＶＣ）的三个分离毒素：ＳＶＣⅠ、ＳＶＣⅡ及ＳＶＣⅢ对Ｅｃａ１０９细胞株的杀伤及生长抑制作用。结果表明，ＳＶＣⅠ和ＳＶＣⅡ对Ｅｃａ１０９细胞有直接杀伤作用，它们对Ｅｃａ１０９细胞的半数杀伤浓度（ＬＣ＿（５０））分别为７．９９９μｇ／ｍｌ、４．４９９μｇ／ｍｌ；ＳＶＣⅡ和ＳＶＣⅢ处理细胞９６ｈ后，对Ｅｃａ１０９细胞的生长抑制率（ＩＲ％）分别达８３．４７％和９２．２９％；ＳＶＣⅡ、ＳＶＣⅢ对Ｅｃａ１０９细胞的集落抑制率分别达８１．３％、８０．１％，它们对Ｅｃａ１０９细胞的半数抑制浓度（ＩＣ＿（５０））分别为９．９００μｇ／ｍｌ、３．０９９μｇ／ｍｌ；经ＳＶＣⅡ、Ⅲ作用后，Ｅｃａ１０９细胞有丝分裂指数（ＭＩ％）亦明显减少；ＭＴＴ法的实验结果显示，ＳＶＣⅡ、Ⅲ对Ｅｃａ１０９细胞具有明显的细胞毒性作用。未观察到ＳＶＣⅠ对Ｅｃａ１０９细胞的作用。
The lethal and the growth-inhibiting effect of three components (SVC Ⅰ,SVCⅡ,SVCⅢ)isolated from scorpion venom on Eca109 cells were studied. The resultsshowed that SVC Ⅱ and SVC Ⅲ possess killing effect on Eca109 cells,the half lethalconcentration(LC_(50) )of the SVC Ⅱ and SVC Ⅲ on Eca109 cells were 7.999μg/ml and 4.499μg/ml respectively. Treated the cell with SVC Ⅱ or SVC Ⅲ for 96 hours,the growth inhi-biting-rate on Eca109 cells were 83.47% and 92.29% respectively. The colony-forminginhibiting rate of SVC Ⅱ and SVC Ⅲ on Eca109 cells were 81.3% and 80.1%,and the halfinhibiting-concentration(IC_(50))were 9.900μg/ml and 3.099μg/ml respectively. The mitoticindex(MI‰)of the Eca109 cells were decreased obviously after the cells were treatedby SVCⅡ and SVC Ⅲ. The cytotoxic effect of SVC Ⅱ and SVC Ⅲ on Eca109 cells was ob-served by means of MTT test.The effect of SVCⅠ on Eca109 cells was not found.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1995年第3期251-255,共5页
Chinese Journal of Pathophysiology
