摘要
目的:研究早发性卵巢功能不全动物模型的造模特点,为早发性卵巢功能不全动物模型制备的规范化提供参考,为该病发病机制及诊疗研究提供更好的研究基础。方法:通过检索中国知网、万方、维普、中国生物医学文献及PubMed数据库获取近十年早发性卵巢功能不全动物实验文献,归纳实验动物种类、造模方式、给药剂量、给药方案、成模标准及检测指标,应用Excel软件进行频数分析,应用SPSS Modeler 18.0进行关联规则分析并运用Cytoscape 3.6.1对结果进行可视化升级。结果:共纳入文献281篇,建立早发性卵巢功能不全动物模型多选用SD大鼠或BALB/c小鼠,造模方式多选用医源性诱导,造模药物以环磷酰胺为主,成模标准多选用动情周期紊乱,检测指标多选用卵巢组织形态学变化、雌二醇、卵泡刺激素及动情周期以从多方面综合评价模型。结论:早发性卵巢功能不全动物实验多选用SD大鼠,以环磷酰胺首次腹腔注射给药50 mg·kg^(-1),第2天起以8 mg·kg^(-1)·d^(-1)维持14 d诱导造模,具有简便易行、成模率高且符合疾病发生过程等优点,可为该病基础动物实验研究提供参考。
Objective:To study the modeling characteristics of the animal model of premature ovarian insufficiency and provide references for the standardization of the animal model of premature ovarian insufficiency,thus offering a better research basis to the pathogenesis,diagnosis,and treatment of the disease.Method:The animal experimental literature of premature ovarian insufficiency in the past decade was obtained by searching China National Knowledge Infrastructure(CNKI),Wanfang,VIP Chinese Technology Periodical Database,China Biology Medicine(CBM),and PubMed.The types of experimental animals,modeling methods,dosage,administration scheme,modeling standards,and detection indicators were summarized.The frequency analysis was performed with Excel,the association rule analysis was performed with SPSS Modeler 18.0,and the results were visually upgraded with Cytascape 3.6.1.Result:A total of 281 articles were included,and most animal experiments on premature ovarian insufficiency were performed on SD rats or BALB/c mice.Most modeling methods were iatrogenic induction,and the main modeling drug was cyclophosphamide.Erestrous cycle disorder was selected as the modeling standard.Ovarian histomorphology,estradiol,follicle stimulating hormone,and estrous cycle were selected as the detection indicators to comprehensively evaluate the model from multiple aspects.Conclusion:SD rats are mostly used to induce modeling in animal experiments on premature ovarian insufficiency through first intraperitoneal injection of cyclophosphamide 50 mg·kg^(-1) and 8 mg·kg^(-1)·d^(-1) from the next day for 14 d,which has the advantages of easy operation,high modeling rate,and consistency with the disease pathogenesis.This paper can provide references for basic animal experimental research of premature ovarian insufficiency.
作者
王艳辉
唐丽
雷磊
WANG Yanhui;TANG Li;LEI Lei(College of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第9期225-233,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖南省自然科学基金项目(2021JJ30493)
湖南省中医药科研计划重点项目(2021007)
湖南中医药大学校级科研基金项目(2020XJJJ001)。
作者简介
第一作者:王艳辉,在读博士,从事中医药防治妇科疾病研究,E-mail:2300652085@qq.com;通信作者:雷磊,教授,博士生导师,从事中医药防治妇科疾病研究,E-mail:leilei1398@sina.com。
