摘要
目的观察滋肾安坤颗粒治疗免疫性早发性卵巢功能不全(POI)小鼠疗效及对白细胞介素2(IL-2)、白细胞介素6(IL-6)、转化生长因子β(TGF-β)的影响。方法筛选动情周期规律的雌性小鼠,以小鼠透明带3(ZP3)为抗原复制免疫性POI小鼠模型。设滋肾安坤颗粒低、高剂量进行治疗,以戊酸雌二醇为阳性对照。巴氏染色法观察动情周期,HE染色法观察卵巢组织形态学变化,ELISA法检测血清雌二醇(E2)、促卵泡激素(FSH)、抗苗勒管激素(AMH)、IL-2、IL-6、TGF-β水平。结果与空白对照组比较,模型组小鼠动情周期延长紊乱,卵巢间质炎性细胞浸润,生长卵泡减少,闭锁卵泡增多(P<0.05,P<0.01);血清E2、AMH、TGF-β水平降低(P<0.01),血清FSH、IL-2、IL-6水平升高(P<0.01)。与模型组比较,各治疗组动情周期紊乱、炎性细胞浸润有改善,其中滋肾安坤高剂量组生长卵泡增加,戊酸雌二醇组闭锁卵泡减少(P<0.05);滋肾安坤高剂量组、戊酸雌二醇组血清E2、AMH、TGF-β水平升高(P<0.05,P<0.01),滋肾安坤高剂量组血清FSH、IL-6水平降低(P<0.05),各治疗组血清IL-2水平降低(P<0.05,P<0.01)。结论滋肾安坤颗粒可改善免疫性POI小鼠血清生殖激素和IL-2、IL-6、TGF-β水平,降低卵巢炎性反应,调节免疫紊乱,提高卵巢储备能力,发挥对卵巢的保护作用。
Objective To observe efficacy of Zishen Ankun Granules in treatment of mice with immune premature ovarian insufficiency(POI)and its influence on cytokines interleukin 2(IL2),interleukin 6(IL6)and transforming growth factor-β(TGF-β)Methods Screening female mice with regular estrus to establish immune POI mice model with zona pellucida 3(ZP3)as antigen.Low and high doses of Zishen Ankun Granules were set up for treatment,and estradiol valerate was used as a positive control.Observing the estrous cycle of mice by papanicolaou staining,observing the morphological changes of ovarian tissues by HE staining,and detecting serum estradiol(E2),follicle-stimulating hormone(FSH),anti mullerian hormone(AMH),IL2,IL6 and TGF-βlevels by ELISA.Results Compared with the blank control group,the estrous cycle of model group was prolonged and disordered,inflammatory cell were infiltrated in the ovarian stroma,growth follicles were decreased,and atresia follicles were increased(P<0.05,P<0.01);serum E2,AMH and TGF-βlevels were decreased(P<0.01),serum FSH,IL2 and IL6 levels were increased(P<0.01).Compared with model group,the estrous cycle disorder and inflammatory cell infiltration were improved in each treatment group,in which the growing follicles increased in the high dose group of Zishen Ankun and the atretic follicles decreased in the estradiol valerate group(P<0.05);serum E2,AMH and TGF-βlevels were increased in Zishen Ankun high dose group and estradiol valerate group(P<0.05,P<0.01),Serum FSH and IL6 levels in Zishen Ankun high-dose group were decreased(P<0.05),Serum IL2 levels was decreased in each treatment group(P<0.05,P<0.01).Conclusion Zishen Ankun granules can improve the levels of serum reproductive hormone,IL2,IL6 and TGF-βin immune POI mice,reduce the inflammatory response of ovary,regulate immune disorders,improve the ovarian reserve capacity,and play a protective role on the ovary.
作者
魏杜燃
彭桃
魏绍斌
吴扬
李茂雅
罗丹
Wei Duran;Peng Tao;Wei Shaobin;Wu Yang;Li Maoya;Luo Dan(School of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China;Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610032,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第7期2628-2634,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
成都中医药大学附属医院科技发展基金课题医院制剂专项(2017-D-YY-75):医院制剂滋肾安坤颗粒的开发研究,负责人:魏绍斌。
作者简介
通讯作者:魏绍斌,教授,主任医师,硕博士研究生导师,主要研究方向:中医药防治妇科疾病。
