摘要
目的基于偏最小二乘回归法(PLS)研究栀子不同炮制品化学成分与肝肾毒性的关联度。方法采用超高效液相色谱法测定生栀子、炒栀子、姜栀子、焦栀子、栀子炭不同炮制品中栀子苷、京尼平龙胆双糖苷、栀子苷酸、去乙酰车叶草酸甲酯、西红花苷I和西红花苷Ⅱ6种化学成分的含量,栀子不同炮制品水提液(7.5 g/kg)连续3 d大鼠ig给药,测定各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、血清肌酐(CREA)、尿素氮(BUN)的含量,使用Origin 9.1软件中的PLS算法分析栀子不同炮制品化学成分与肝肾毒性的关联度。结果栀子炮制后栀子苷、西红花苷I和西红花苷Ⅱ的含量不同程度的降低,京尼平龙胆双糖苷、去乙酰车叶草酸甲酯和栀子苷酸含量在部分炮制品中升高;栀子及炮制品对正常大鼠可造成不同程度的肝、肾损伤(生栀子>炒栀子>姜栀子>焦栀子>栀子炭);PLS分析表明6种主要化学成分中栀子苷与西红花苷I的变量重要性值均超过0.8。结论栀子苷与西红花苷I可能是栀子肝肾毒性的物质基础,通过炮制降低栀子肝肾毒性的作用可能与炮制后栀子苷与西红花苷I含量降低有关。
Objective To explore the relationship between the chemical constituents and liver and kidney toxicity of different processed Gardenia jasminoides products based on partial least square regression. Methods The contents of chemical constituents in5 processed products of Gardenia jasminoides were determined by ultra performance liquid chromatography, water extracts of different processed Gardenia jasminoides products(7.5 g/kg) were administered by gavage to rats for three consecutive days, and the contents of ALT, AST, TBIL, CREA, and BUN in serum were determined at the last day, the correlation between chemical constituents and liver and kidney toxicity of different processed Gardenia jasminoides products were analyzed using the PLS algorithm in the Origin 9.1 software. Results The contents of geniposide, crocetin I and crocetin II decreased to varying degrees after processed, but the contents of genipin-1-β-D-gentiobioside, geniposidic acid,deacetyl asperulosidic acid methyl esterin were higher than those in raw Gardenia jasminoides. Five kinds of processed Gardenia jasminoides products caused different liver and kidney damage on normal rats(raw Gardenia jasminoides > fried Gardenia jasminoides > ginger Gardenia jasminoides > scorched Gardenia jasminoides > Gardenia jasminoides charcoal). PLS analysis showed the variable importance values of geniposide and crocetin I among the six main chemical components exceed 0.8. Conclusion Geniposide and crocetin I may be the material basis of Gardenia jasminoides which caused liver and kidney toxicity. The effect of reducing liver and kidney toxicity caused by processing may be related to the decrease of the contents geniposide and crocetin I.
作者
李会芳
刘静婷
郎霞
路晓娟
LI Huifang;LIU Jingting;LANG Xia;LU Xiaojuan(College of Traditional Chinese Medicine and Food Engineering,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China)
出处
《药物评价研究》
CAS
2021年第9期1890-1896,共7页
Drug Evaluation Research
基金
国家自然科学基金(81903913)。
关键词
栀子
栀子苷
西红花苷I
肝肾毒性
偏最小二乘法分析
Gardenia jasminoides Ellis.
geniposide
crocetin I
hepatorenal toxicity
partial least square
作者简介
通信作者:李会芳,副教授,主要从事中药药理及毒理学研究。Tel:(0351)3179903 E-mail:sunshine_613@163.com。
