摘要
目的建立栀子不同炮制品HPLC指纹图谱分析方法,结合化学模式识别技术为栀子的炮制提供参考。方法采用Phenomenex Luna-C_(18)色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.08%甲酸水溶液为流动相,梯度洗脱;流速:0.85 mL·min^(-1);检测波长:240,254,280,330,440 nm,柱温:35℃,进样量:10μL。采用相似度评价、聚类分析和正交偏最小二乘法-判别分析评价20批栀子不同炮制品指纹图谱。结果建立了栀子不同炮制品指纹图谱,共标定17个共有峰,经与对照品比对指认出其中8个化合物。20批栀子相似度为0.901~0.969,通过聚类分析可将样品聚为3类,结合正交偏最小二乘法-判别分析发现5个成分可能是造成栀子不同炮制品差异性的主要差异标志物。结论本研究建立的HPLC指纹图谱结合化学模式识别技术能够反映不同炮制方法栀子的差异,可为栀子的炮制提供参考。
Objective To establish an HPLC fingerprint analysis method of different processed products of Gardeniae Fructus,and to provide reference for processing Gardeniae Fructus combined with chemical pattern recognition technology.Methods The analysis was performed on Phenomenex Luna-C_(18)(250 mm×4.6 mm,5μm)column with mobile phase consisted of acetonitrile-0.08%formic acid aqueous solution(gradient elution)at the flow rate of 0.85 mL·min^(-1),the detection wavelength were set at 240,254,280,330,440 nm,the column temperature was 35℃,the sample injection volume was 10μL.The fingerprint of 20 batches of Gardeniae Fructus was evaluated by similarity evaluation,cluster analysis(CA)and orthogonal partial least square discriminate analysis(OPLS-DA).Results HPLC fingerprint of Gardeniae Fructus was established successfully and 17 common peaks were identified in the study,eight chemical compounds were identified comparison with standard references.The similarity of 20 batches were 0.901-0.969,the samples were classified into three groups by CA combined with OPLS-DA,five components were the main markers that cause differences in the different processed products of Gardeniae Fructus.Conclusion The HPLC fingerprint combined with chemical pattern recognition technology established in this study can reflect the differences of Gardeniae Fructus in different processing methods,and can provide reference for the processing of Gardeniae Fructus.
作者
施文文
楼云雁
Wen-Wen SHI;Yun-Yan LOU(Department of Pharmary,Hangzhou Cancer Hospital,Hangzhou 310000,China)
出处
《中国药师》
CAS
2023年第10期44-50,共7页
China Pharmacist
关键词
栀子
高效液相色谱指纹图谱
相似度
化学模式识别
聚类分析
正交偏最小二乘法-判别分析
Gardeniae Fructus
HPLC fingerprint
Similarity
Chemical pattern recognition
Cluster analysis
Orthogonal partial least square discriminate analysis
作者简介
通信作者:施文文,初级药师,Email:henay69486@163.com。
