摘要
目的:基于UPLC-MS/MS法同时测定栀子中14个萜类成分的含量。方法:以70%乙醇为提取溶剂,采用ACQUITY UPLC^(TM)BEH C_(18)(100 mm×2.1 mm,1.8μm)色谱柱;以含0.1%甲酸的乙腈-0.1%甲酸溶液为流动相,梯度洗脱;流速为0.6 mL/min;柱温为40℃;进样量为1.0μL。14个萜类成分中去乙酰车叶草苷酸甲酯、山栀苷、京尼平、京尼平-1-O-β-D-龙胆双糖苷、山栀子苷B、京尼平苷酸、栀子苷、鸡矢藤次苷甲酯、栀子新苷、西红花苷Ⅰ、西红花苷Ⅲ、藏红花酸采用负离子检测模式,山栀苷甲酯和西红花苷Ⅱ采用正离子检测模式。结果:栀子中上述14个萜类成分的方法学考察结果良好。其中,栀子苷、去乙酰车叶草苷酸甲酯和京尼平-1-O-β-D-龙胆双糖苷在栀子中含量较高,不同产地的栀子中14个萜类成分含量表现出一定差异。结论:该方法准确,重复性好,检测限低,能够客观反映不同产地栀子药材的主要萜类成分含量差异。
Objective:To establish an UPLC-MS/MS method for the simultaneous determination of the contents of 14 terpenoids in Gardeniae Fructus.Methods:The 70%ethanol was used as extraction solvent.The ACQUITY UPLC^(TM)BEH C_(18)(100 mm×2.1 mm,1.8μm)column was used,mobile phase was acetonitrile containing 0.1%formic acid-0.1%formic acid with gradient elution,flow rate was 0.6 mL/min,column temperature was 40℃,injection volume was 1.0μL.Among the 14 terpenoids,6-α-hydroxygeniposide,shanziside,genipin,genipin-1-O-β-D-gentiobioside,shanziside B,geniposidic acid,geniposide,scandioside methyl ester,gardoside,crocinⅠ,crocinⅢ,crocetin were detected under negative ion detection mode,and shanziside methyl ester and crocinⅡwere detected under positive ion detection mode.Results:The methodological investigation results of 14 terpenoids were good.Geniposide,6-α-hydroxygeniposide and genipin-1-O-β-D-gentiobioside had the higher contents in Gardeniae Fructus.The contents of 14 terpenoids in Gardeniae Fructus from different habitats showed certain differences.Conclusion:The method is accurate,reproducible and has low detection limit,which can objectively reflect the differences of main terpenoids in Gardeniae Fructus from different habitats.
作者
周聪
周利
李翔
熊丰
杨健
张小波
ZHOU Cong;ZHOU Li;LI Xiang;XIONG Feng;YANG Jian;ZHANG Xiao-bo(Academician Workstation,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Chinese Medicine Resource Center,China Academy of Chinese Medical Sciences/State Key Laboratory Breeding Base of Dao-di Herbs,Beijing 100700,China;Jiangxi Research Center for Quality Evaluation of Dao-di Herbs,Ganjiang New District 330000,China)
出处
《中药材》
CAS
北大核心
2022年第7期1644-1649,共6页
Journal of Chinese Medicinal Materials
基金
中国中医科学院科技创新工程(CI2021A03901)
国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202005)
国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004)
中央本级重大增减支项目(2060302)
赣江新区科技计划项目(赣新管创发(2020)26号)
作者简介
周聪(1995-),男,在读硕士研究生,专业方向:中药资源及化学分析,Tel:18131376536,E-mail:zhoucong3366@163.com;通讯作者:张小波,Tel:010-64081790,E-mail:jack110007@163.com。
