摘要
目的通过观察心痛方对急性心肌梗死大鼠SERCA2a mRNA表达及心律失常的影响,探讨心痛方防治急性心肌梗死后心律失常的疗效机制。方法 40只SD大鼠分为假手术组、模型组、心痛方组、硫氮酮组。采用结扎法制备心肌梗死模型,运用RT-PCR法测定SERCA2a mRNA表达,结扎后心电图检测心律失常。结果心痛方组能显著增加SERCA2a mRNA表达(P<0.05),降低心律失常发生率(P<0.05)。结论心痛方对急性心肌梗死大鼠具有保护作用,其作用机制可能与增加SERCA2a mRNA的表达,从而降低心律失常发生有关。
Objective： To observe the effect of Xintongfang on the expression of SERCA2a mRNA and arrhythmia in acute myocardial infarction rats to explore the therapeutic mechanism of Xintongfang on arrhythmia after acute myocardial infarction. Methods： 40 SD rats were randomly divided into sham operation group,model group,Xintongfang group and diltiazem control group. Acute myocardial infarction model was established by coronary artery ligation method. Expression of SERCA2a mRNA was observed by RT-PCR method and arrhythmia was detected by ECG. Results： The expression of SERCA2a mRNA was obviously increased in Xintongfang group （P 〈 0.05 ） ;and also the incidence of arrhythmia was decreased （P 〈 0.05 ）. Conclusion： Xintongfang has a protective effect on acute myocardial infarction in rats. The mechanism of the effect may be related to its increasing of expression of SERCA2a mRNA in rat,which can help decrease the incidence of arrhythmia.
出处
《中国中医急症》
2017年第1期30-32,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
湖南省自然科学基金项目(13jj5031)
湖南省教育厅科学研究项目(16A159)
作者简介
通信作者（电子邮箱：fanjr218@sina．com）
