摘要
【目的】探讨冠心病痰瘀互结证动物模型的复制方法。【方法】采用中西医结合及病证结合方法造模,根据症状、体征和指标变化,及祛瘀化痰中药反证以判断模型成功与否。Wistar大鼠60只,随机分为正常组,模型组和血府逐瘀汤合瓜蒌薤白半夏汤低、高剂量组(中药低、高剂量组);正常组基础饲料喂养,每日予以等容积生理盐水灌胃;其他组均饲以高脂饲料和脂肪乳液(10 mL.kg-1.d-1),中药低、高剂量组分别以12、24 g.kg-1.d-1剂量灌服血府逐瘀汤合瓜蒌薤白半夏汤悬液,模型组灌服等容积生理盐水,连续7周,并于第35天施冠状动脉结扎术。观察各组造模前、后的心电图改变;检测血脂、心肌酶及血液流变学指标;处死动物后取心脏,称质量计算心脏指数。【结果】模型组心电图显示心肌严重缺血,而中药可改善心肌缺血;模型组胆固醇、甘油三酯、肌酸激酶、全血比粘度、红细胞压积及心脏指数均比正常组升高(P<0.001),而中药低、高剂量组上述指标均比模型组有不同程度的改善(P<0.001)。【结论】采用高脂饮食加冠状动脉结扎术方法复制的动物模型与人类痰瘀互结证的冠心病特征相似,并以祛瘀化痰药物治疗有效,说明模型复制成功;该造模方法操作简便,重复性好,适用于对冠心病痰瘀互结证的研究。
[Objective] To explore a method for the establishment of animal model of coronary heart disease （CHD） with the blending of phlegm and blood stasis. [Methods] Methods of TCM-WM combination （traditional Chinese medicine integrated with westem medicine） and disease-syndrome combination were used to establish a model. The model was evaluated by the changes of symptoms, signs and laboratory indexes and by the effect of blood-stasis-removing and phlegm-resolving herbs on the model. Sixty Wistar rats were randomized into normal group, model group, low-dose herbal medicine group and high-dose herbal medicine group. The normal group was fed with routine forage and normal saline, and the other three groups with high-fat forage and fat milk （10mL·kg^-1·d^-1）. Low- and high-dose herbal medicine groups were given Xuefu Zhuyu Decoction combined with Gualou Xiebai Banxia Decoction in the dosages of 12 and 24 10g·kg^-1·d^-1 respectively by gastric gavage, and the model group given the same volume of normal saline for 7 consecutive weeks. Coronary artery ligation was performed in the 35th day. The changes of electrocardiogram （ECG） were observed before and after the modeling, and blood lipid, myocardial enzymes and hemorrheological parameters were determined. The heart weight was examined to calculate heart index. [Results] ECG in the model group indicated severe myocardial ischemia, which may be relieved by herbal medicine. Serum contents of cholesteril, tryglyceride and creatine kinase （CK）, whole blood viscosity, hematocrit and heart index were obviously increased in the model group, while the above indexes decreased to different degrees in herbal medicine groups （P 〈 0.001）. [Conclusion] The manifestations of the CHD rat model are similar to those of the CHD in patients, indicating the success of model establishment. This method is simple and reproducible, and suited for the research of CHD with the blending of phlegm and blood stasis.
出处
《广州中医药大学学报》
CAS
2006年第4期346-349,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
关键词
冠状动脉疾病
模型
心血管
模型
动物
证候
大鼠
CORONARY DISEASE
MODELS, CARDIOVASCULAR
MODELS, ANIMAL
SYNDROME COMPLEX
RATS
作者简介
朱丽红（1981-），女，医学硕士
