摘要
目的:建立黄芩的超高效液相色谱-电喷雾-飞行时间质谱(UPLC-ESI-TOF/MS)分析方法,研究黄芩的化学成分。方法:分别采集UPLC-TOF/MS全扫描质量色谱图和源内裂解的UPLC-TOF/MS色谱图,通过分子离子精确质量和同位素拟合度的分析确定分子式,在数据库中检索匹配的化合物,进而解析源内裂解的质谱图,从而对各峰进行鉴定或结构表征。结果:共鉴定或表征了黄芩药材中31个成分,均为黄酮类化合物。结论:该方法快速、灵敏、准确,可用于黄芩化学成分的分析,也为其它中药的成分分析提供了思路。
Objective ： A method using ultra performance liquid chromatography - electrospray ionization - time of flight mass spectrometry （UPLC -ESI -TOF/MS） was developed to analyze the constituents in Scutellariae Radix. Methods：The full- scan UPLC -TOF/MS chromatogram and the chromatogram produced using in - source collision -induced dissociation were acquired, respectively. Through calculating accuracy mass and i- sotopic fit value of the molecular ion cluster, molecular formula was confirmed, and further the matched com- pound was researched in the established database composed of the known constituents in Scutellariae Radix. Finally, the peaks were identified or characterized structurally by elucidating the mass spectrum produced u- sing in - source collision - induced dissociation. Results ： As a result, 31 compounds in Scutellariae Radix were detected, all of which were assigned to the flavonoids. Conclusion：It is concluded that this method is rapid, sensitive and accuracy, and can be used for analysis of the constituents in Scutellariae Radix. This study also provides a method to the analysis of other traditional Chinese medicines.
出处
《中医药信息》
2012年第4期20-24,共5页
Information on Traditional Chinese Medicine
基金
国家重点基础研究发展(973)计划(2007CB512608)
哈尔滨市科技创新人才研究专项(RC2008XK003009)
关键词
黄芩
UPLC-ESI-TOF/MS
成分分析
高分辨质谱
源内裂解
Scutellariae Radix
UPLC - ESI - TOF/MS
Constituents analysis
High - resolution mass spec- trometry
In - source collision - induced dissociation
作者简介
刘大伟（1956-），男，高级讲师，主要从事生物医学及生药学研究。
通讯作者：刘树民（1963-），男，博士，教授，博士研究生导师，主要从事中药临床药效物质基础及中药药性理论。
