摘要
目的:应用超高效液相色谱-四级杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)技术鉴定白毛银露梅中的主要化学成分。方法:采用AccucoreaQ RP18(2.1 mm×150 mm, 2.6μm)色谱柱,以甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱,流速0.3 mL/min,柱温25℃。质谱条件采用高效电喷雾离子源(HESI),在负离子模式下,一级全扫描/数据依赖型二级质谱扫描模式检测,分析鉴定白毛银露梅中的主要化学成分。结果:结合高分辨质谱提供的一级准分子离子峰和二级碎片信息,参照相关文献,以及对照品比对,共鉴定出36个化学成分,包括19个黄酮类成分、9个有机酸类成分、6个儿茶素类成分和2个香豆素类成分,均为首次报道,其中5个成分经对照品进行了验证。结论:该方法可快速分析鉴定白毛银露梅中的化学成分,为合理开发其药用及食用价值提供科学依据。
Objective: To identify the main chemical constituents of Potentilla glabra var.mandshurica based on ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS).Methods: The chromatographic separation was performed on an AccucoreaQ RP18(2.1 mm×150 mm, 2.6 μm)column, the mobile phase was methanol-0.1% formic acid solution with gradient elution.The flow rate was 0.3 mL/min, column temperature was 25 ℃.Under the condition of high efficiency electrospray ion source(HESI)and the negative ion mode, the primary full scan/data dependent secondary mass spectrometry scanning mode was used to detect and analyze the main chemical constituents in Potentilla glabra var.mandshurica.Results: Combined with the primary excimer ion peak and secondary fragment information provided by high resolution mass spectrometry, thirty-six components, including 19 flavonoids, 9 organic acids, 6 catechins and 2 coumarins, were identified by referring to relevant literature and comparing with reference materials, both were first reported, and five of them were confirmed by reference standards.Conclusion: The established method can analyze the components in Potentilla glabra var.mandshurica rapidly, which provides evidence for the exploration of medicinal and edible value of this plant reasonably.
作者
崔小敏
万兆新
任慧
胡静
陈娟
孟雪
陈志永
CUI Xiao-min;WAN Zhao-xin;REN Hui;HU Jing;CHEN Juan;MENG Xue;CHEN Zhi-yong(Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,China;Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi'an 710003,China)
出处
《中药材》
CAS
北大核心
2020年第8期1901-1906,共6页
Journal of Chinese Medicinal Materials
基金
陕西省中医药管理局项目(2019-ZZ-ZY020)
陕西省中医药研究院项目(2018-05)
关键词
白毛银露梅
药王茶
化学成分
轨道阱高分辨质谱
黄酮
Potentilla glabra var.mandshurica(Maxim.)Hand.-Mazz.
Yaowang tea
Chemical constituents
Orbitrap HRMS
Flavonoids
作者简介
崔小敏(1986-),女,硕士,助理研究员,研究方向:中药药效物质基础研究,Tel:029-85395696,E-mail:cuixiaomin406@163.com;通讯作者:陈志永,Tel:029-85395696,E-mail:18829014325@163.com。
