摘要
目的:建立黄芩中主要黄酮类成分的高效液相色谱-二极管阵列检测器(HPLC-DAD)含量测定方法,并研究产地、生长年限、采收期、栽培和加工方法等对主要黄酮类成分含量的影响。方法:采用Thermo ODS-2 Hypersil色谱柱(4.6 mm×250mm,5μm),以乙腈-0.1%磷酸水-四氢呋喃为流动相进行梯度洗脱,检测波长274 nm,流速1.00 mL.min-1,柱温26℃,进样量20μL。结果:6个主要黄酮,黄芩苷(1)、千层纸素A苷(2)、汉黄芩苷(3)、黄芩素(4)、汉黄芩素(5)和千层纸素A(6),分别在选定范围内线性关系良好(R2≥0.999 3),平均加样回收率介于96.6%~103.0%,RSD均小于5.0%(n=9)。结果:不同产区黄芩质量差异很大。传统道地和传统非道地黄芩药材中黄酮含量没有显著性差异,但现在道地与非道地、传统道地与现在道地黄芩药材之间化合物1,2以及6个黄酮含量之和存在较显著差异;生长年限对药材质量影响不是特别显著,但一、二年生药材中的黄酮含量稍高;春季采收比秋季采收的黄芩黄酮含量高;野生与栽培药材中的黄酮含量除化合物3,6外,其他没有显著性差异;与药材比较商品饮片中黄酮苷的含量较低,而黄酮苷元的含量则较高。结论:本法简便、准确、重现性好,可作为黄芩药材质量检测的方法。
Objective： To develop a HPLC-DAD method for simultaneous determination of six flavonoids in Scutellaria baicalensis, and study the effect of geographic sources, cuhuration, harvesting time and processing on the contents of flavonoids. Method： The analysis was performed on a Thermo ODS-2 Hypersil column （4. 6 mm×250 mm, 5 μm） at 26 ℃with a gradient program of acetonitrile-0. 1% H3PO4 aqueous solntion-tetrahydrofuran as the mobile phase and a Diode Array Detector as the detector with the detection wavelength at 274 nm. The flow rate was 1.0 mL· min-1, and the injection value was 20 p^L. Result： Six flavonoids, baicalin （1）, oroxylin A-7-O-glucnronide （2）, wogonoside （3）, baicalein （4）, wogonin （5） and oroxylin A （6）, were linear over the selected range with R2≥0. 999 3. The average recoveries were between 96. 6% -103.0%, and RSD were less then 5.0% （n =9） for flavonoids 1-6. By analyzing the data obtained, the effect of geographic sources, culturation, harvesting time and processing on the contents of flavonoids were discussed. Conclusion： This method is simple, sensitive, reliable and reproducible. It can be used for the quality control of S. baicalensis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2910-2915,共6页
China Journal of Chinese Materia Medica
基金
国家基础研究发展计划(973)项目(2006CB504-707)
关键词
黄芩
黄酮
含量测定
HPLC-DAD
Scutellaria baicalensis
flavonoids
quality control
HPLC-DAD
作者简介
[通信作者]张庆英，Tel：（010）82801725，Fax：（010）82801592，E—mail：qyzhang@hsc．pku．edu．cn
