摘要
本研究用不同浓度人参皂甙Rg1作用人胃癌BGC-823细胞24 h、48 h和72 h,采用MTT法、流式细胞术及半定量RT-PCR检测GS-Rg1对胃癌细胞的增殖抑制作用、细胞周期分布时相和p16^(INK4a)、p21^(WAF1)表达水平的影响,以探讨人参皂甙Rg1对人胃癌BGC-823细胞增殖的抑制作用及机制。结果表明,随着作用时间和浓度的增加,人参皂甙Rg1对胃癌细胞增殖抑制作用逐渐增强(P<0.05),G_0/G_1期细胞比例增加,G_2/S期细胞比例下降,p16^(INK4a)、p21^(WAF1)基因水平上调。上述结果提示人参皂甙Rg1能抑制体外培养的胃癌BGC-823细胞增殖,其机制可能与上调肿瘤细胞内细胞周期蛋白依赖激酶抑制因子p16^(INK4a)及p21^(WAF1)mRNA的表达有关。
To explore the inhibitory effects of ginsenoside Rgl on the human gastric carcinoma cell line BGC-823 in vitro, 24, 48 and 72 h after treated with different concentration of GS-Rgl, the cell proliferation of the BGC-823 cells was valuated by MTT assay, cell cycle distribution was measured by flow cytometric analysis and the expression of p16^INK4a and p21^WAF1 genes mRNA were detected by reverse transcription PCR （RT-PCR）. Results showed that with the increase of concentration of GS-Rgl and the duration of administration, the inhibitory effects were elevated （P〈0.05）. The proportion of cells in G0/G1 phase was obviously increased whereas that in G2/S phase was decreased. The expression ofpl6^INK4a and p21^WAF1 genes mRNA were upregulated. These results suggested that GS-Rgl can inhibit the proliferation of BGC-823 cells in vitro by upregulating the expression ofpl6^INKga and p21^WAFI genes mRNA.
出处
《中国细胞生物学学报》
CAS
CSCD
2011年第3期269-274,共6页
Chinese Journal of Cell Biology
作者简介
通讯作者。Tel：13618393893，E·mail：caiquanzhang@foxmail．com
