摘要
目的 :前瞻性研究抑癌基因P1 6蛋白在人胃癌及转移淋巴结中的表达与胃癌转移的关系。方法 :应用SP免疫组织化学方法检测 46例原发性胃癌及 6 9枚转移淋巴结中P1 6蛋白表达。结果 :P1 6蛋白在受检原发性胃癌中的阳性率分别为 1 7.4% (8/46 ) ,在转移淋巴结中的阳性率分别为 1 5 .9% (1 1 /6 9)。原发癌伴淋巴结转移组P1 6的阳性表达率 (6 .9% )低于不伴淋巴结转移组的阳性率 (3 5 .3 % ) ,差异有显著性 (P <0 .0 5 )。P1 6表达与肿瘤大小、部位、浸润深度、Bormann分型、年龄及性别等因素无相关性(P >0 .0 5 )。P1 6在原发性胃癌及转移淋巴结中的表达无相关性 (P >0 .0 5 )。结论 :P1 6基因蛋白对胃癌的发展及淋巴结转移有抑制作用。检测胃癌P1 6蛋白对评估胃癌生物学行为、预测胃癌淋巴结转移以及预后可能具有一定的价值。
Objective: To study the role of the P16 oncoproteins in development of human gastric carcinomaand lymph node metastasis prespectively. Methods: The expression of P16 in 46 cases of primary gastric carcinomas and 69 metastatic lymph nodes was detected by SP immunohistochemical method. All samples were obtained freshly,fixed with 10% buffered formalin immediately ,embedded with parafin and then sectioned serially.Using konwn positive staining section of P16 in esophageal carcinoma as control.Results:The positive expression rates of P16 were 17.4% in primary gastric carcinomas and 15.9% in metastatic lymph nodes respectively .The positive rate of P16 in primary gastric carcinomas with lymph onde metastasis was lower than that in ones without lymph node metastasis(P<0.05).No correlations was found between the expressions of P16 in primary gastric carcinomas and metastatic lymph nodes.Conclusions:The P16 oncoproteins might play an important role in suppression of gastric carcinoma metastasis.the detection of P16 oncoproteins might reflect the the biological behaiour,lymphatic metastasis and prognosis of gastriccarcinoma.
出处
《长治医学院学报》
2001年第3期161-163,共3页
Journal of Changzhi Medical College
关键词
胃癌
抑癌基因
P16
免疫组织化学
转移淋巴结
gastric carcinoma
Tumor supressor gene
P16
Immunohistochemistry
Metastatic lymph node
