摘要
目的了解惠州市儿童EB病毒(EBV)与人巨细胞病毒(HCMV)感染情况,为感染防控和临床治疗提供参考依据。方法收集901例住院患儿的临床资料和外周血样本,用荧光定量PCR法检测外周血单个核细胞(PBMC)中的病毒核酸,用酶联免疫吸附试验检测血清中病毒抗体,按年龄进行分组,比较不同年龄段病毒感染阳性率。结果901例患儿的临床疾病诊断中肺炎发生率最高(27.5%),其次为传染性单核细胞增多症(21.0%)、上呼吸道感染(15.9%)和支气管炎(12.5%)。患儿的EBV-DNA和EBV-IgM抗体检测总阳性率分别为61.7%(556/901)和17.5%(158/901),其中学龄前组EBV感染阳性率最高,婴儿组阳性率最低,差异有统计学意义(P<0.05)。6岁及以下患儿随着年龄的增长,EBV感染阳性率逐步增加,而6岁以上患儿的阳性率明显下降,1~6岁患儿EBV感染人数占总阳性样本的74.6%(415/556)。HCMV-DNA和HCMV-IgM抗体检测总阳性率分别为26.9%(242/901)和9.7%(87/901),其中婴儿组HCMV感染阳性率最高,学龄组阳性率最低,随着年龄的增长阳性率逐步降低(P<0.05),0~3岁患儿HCMV感染人数占总阳性样本的83.9%(203/242)。6.3%的患儿存在合并感染,幼儿组居多。结论EBV和HCMV感染有年龄分布特点和合并感染现象存在,早期快速的病原体实验室诊断有助于临床合理治疗与疾病预防控制。
Objective To investigate the infection of Epstein-Barr virus(EBV)and human cytomegalovirus(HCMV)in Huizhou children,and provide reference for infection control and prevention and clinical treatment.Methods Clinical data and peripheral blood samples of 901 hospitalized children were collected.Viral nucleic acid in peripheral blood mononuclear cell(PBMC)was detected by fluorescent quantitative PCR;viral antibody in serum was detected by enzyme-linked immunosorbent assay(ELISA).The positive rates of viral infection were compared among different age groups.Results The incidence of pneumonia was the highest(27.5%),followed by infectious mononucleosis(21.0%),upper respiratory tract infection(15.9%)and bronchitis(12.5%).EBV-DNA and EBV-IgM antibody were positive in 61.7%(556/901)and 17.5%(158/901)children,respectively.The highest positive rate of EBV infection was detected in preschool group and the lowest one in infants group(P<0.05).With increasing age of children aged 6-year and under,the positive rate of EBV infection was gradually increased,while the positive rate was significantly decreased in children over 6-year old.The number of EBV infection in children aged 1-6 year old was accounted for 74.6%(415/556)of the total positive samples.HCMV-DNA and HCMV-IgM antibody were positive in 26.9%(242/901)and 9.7%(87/901)children,respectively.The highest positive rate of HCMV infection was detected in infants group and the lowest one in school-age group,and the positive rate gradually decreased with the increase of age(P<0.05).The number of HCMV infection in children aged 0-3 year old was accounted for 83.9%(203/242)of the total positive samples.6.3%children had co-infection,and co-infection patients were mostly young children group.Conclusion EBV and HCMV infection had the characteristics of age distribution and co-infection.Early rapid laboratory diagnosis of pathogens should be helpful for rational clinical treatment and disease control and prevention.
作者
王春晖
曹碧红
吴显劲
李旭艳
WANG Chun hui;CAO Bi hong;WU Xian jin;LI Xu yan(Inspection Center of Huizhou Municipal Central Hospital,Huizhou,Guangdong 516001,China)
出处
《热带医学杂志》
CAS
2019年第11期1351-1353,1361,共4页
Journal of Tropical Medicine
基金
惠州市科技计划项目(2018Y027).
关键词
儿童
EB病毒
人巨细胞病毒
感染
Children
EB virus
Human cytomegalovirus
Infection
作者简介
王春晖(1985-),男,硕士,检验主管技师,主要从事临床分子生物学检验相关工作;通讯作者:李旭艳,E mail:48945292@qq.com
