摘要
目的比较“上巨虚”与“天枢”配伍“上巨虚”对IBS模型大鼠心率变异性及血清cAMP、cGMP、SP含量的影响,探讨“合募配穴”调节IBS大鼠自主神经平衡性是否存在协同效应。方法选用雄性清洁级Wistar大鼠55只,随机分为空白组(10只)和造模组(45只),造模组采用急-慢性应激联合制备IBS模型,将制备成功IBS模型大鼠随机分为模型组(10只)、单穴组(10只)、合募配伍组(10只);单穴组、合募配伍组连续电针干预14 d;电针治疗后,记录各组大鼠体重、AWR半定量评分、行为学和心率变异性以及检测血清cAMP、cGMP、SP含量。结果干预前,与空白组相比,模型组与电针治疗组大鼠体质量降低,AWR评分升高,大鼠内脏敏感性增加(P<0.05、P<0.01);治疗后,与模型组相比,电针能明显增加大鼠的体质量(P<0.01),降低内脏敏感性(P<0.05、P<0.01),增加大鼠进入开放臂次数、停留时间以及活动轨迹的总路程(P<0.05、P<0.01),降低LF/HF(P<0.05),升高HF以及RMSSD(P<0.05),降低血清cAMP、cGMP、SP含量(P<0.01、P<0.05、P<0.01);电针单穴组与合募配伍组相比,大鼠体质量、AWR评分、行为学、HRV以及血清cAMP、cGMP、SP含量均无统计学差异。结论单穴以及合募配穴均能有效提高IBS内脏敏感性,缓解焦虑负性情绪,调节心率变异性,降低血清cAMP、cGMP、SP含量,但合募配穴并未表现出协同效应。
Objective To compare the effects of"ST37"and"ST25"on heart rate variability and serum cAMP,cGMP and SP levels in rats with IBS model.We investigated whether there was a synergistic effect of the combined acupoints in regulating the autonomic balance of IBS rats.Methods 55 male clean-grade Wistar rats were selected and randomly divided into blank group(10 rats)and modelling group(45 rats),and the modelling group was prepared by acute and chronic combined IBS model,and the successfully prepared IBS model rats were randomly divided into the modelling group(10 rats),the single-point group(10 rats),and the combined-point group(10 rats);the single-point group and the combined-point group were continuously intervened by electroacupuncture for 14 days;after the electroacupuncture treatment,the rats in each group were recorded.After treatment,the body weight,semi-quantitative AWR score,behavioural and HRV measurements,and serum cAMP,cGMP and SP levels were recorded in each group.Results Before intervention,compared with the blank group,rats in the model group and the electroacupuncture treatment group had lower body mass,higher AWR scores,and increased visceral sensitivity(P<0.05,P<0.01);after treatment,compared with the model group,electroacupuncture significantly increased body mass(P<0.01),decreased visceral sensitivity(P<0.05,P<0.01),increased the number of times the rats entered the open arm,the residence time,and total distance travelled by the activity trajectory(P<0.05,P<0.01),decreased LF/HF(P<0.05)and elevated HF as well as RMSSD(P<0.05),and decreased serum cAMP、cGMP and SP content(P<0.01,P<0.05,P<0.01);there were no statistical differences in body mass,AWR score,behavioural,HRV,and serum cAMP,cGMP and SP content of the rats in the electroacupuncture single-point group compared with the combination and matching group.Conclusion Both the application of single acupoint and combined acupoints have been demonstrated to effectively enhance visceral sensitivity in IBS patients,alleviate anxiety and negative affect,modulate heart rate variability,and decrease the serum levels of cAMP,cGMP,and substance P.but the use of combined acupoints did not exhibit an additive synergistic effect.
作者
刘婷婷
张擎宇
王正文
陈少宗
郭述豪
袁志英
朱明航
屈媛媛
冯楚文
杨添淞
LIU Ting-ting;ZHANG Qing-yu;WANG Zheng-wen;CHEN Shao-zong;GUO Shu-hao;YUAN Zhi-ying;ZHU Ming-hang;QU Yuan-yuan;FENG Chu-wen;YANG Tian-song(Heilongjiang University of Chinese Medicine,Haebin 150040,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Institute of Acupuncture and Moxibustion,Shandong University of Traditional Chinses Medicine,Jinan 250355,China;First Affiliated Hospitcd of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Key Laboratory of Chinese Medicine Informatics in Heilongjiang Province,Harbin 150040,China)
出处
《时珍国医国药》
CSCD
北大核心
2024年第15期3486-3492,共7页
Lishizhen Medicine and Materia Medica Research
基金
国家重点研发计划项目(2019YFC1712105)。
关键词
肠易激综合征
电针
合募配穴
心率变异性
协同效应
Irritable bowel Syndrome
Elecroacupuncture
He-Mu adjunct
Heart rate variability
Synergistic effect
作者简介
刘婷婷(1990-),女(汉族),山东枣庄人,黑龙江中医药大学在读博士研究生,主要从事神经系统疾病及亚健康的中西医结合临床与研究工作;通讯作者:陈少宗(1963-),男(汉族),山东潍坊人,山东中医药大学针灸研究院教授,学士学位,主要从事腧穴作用规律、针刺作用规律的现代针灸学研究与临床工作;通讯作者:王正文(1991-),女(汉族),山东汶上人,山东中医药大学实验师,硕士学位,主要从事现代针灸学研究工作.
