摘要
目的探讨分别对"足三里""内关""太冲"穴电针预处理后对腹泻型肠易激综合征大鼠炎性因子IL-1β、IL-10及5-HT、SIgA等相关物质浓度的影响。方法购雌性清洁级Wistar大鼠孕鼠,待孕鼠生产后将乳鼠随机分为5组,空白组,模型组,足三里组,内关组,太冲组,每组10只。用三因素法(母子分离+醋酸灌胃+束缚应激)制备腹泻型肠易激综合征大鼠模型,造模时间为14 d。空白组正常喂养,不做任何处理;模型组造模后每日进行抓取训练和鼠衣穿脱训练;足三里组造模后电针足三里穴,内关组造模后电针内关穴。太冲组造模后电针太冲穴。电针干预后观察各组大鼠体质量增长率、粪便含水率;记录30 min内各组大鼠结肠蠕动波个数;采用ELISA法测定血清中IL-1β、IL-10、5-HT、SIgA的含量。电镜观察各组大鼠结肠组织变化情况。结果与空白组比较,模型组大鼠粪便含水率与结肠蠕动波增加、血清中致炎因子IL-1β和5-HT明显增加、血清中抑炎因子IL-10和SIgA显著减少,结肠组织超微结构紊乱,微绒毛断裂,排列不齐。差异具有统计学意义(P<0.05);与模型组比较,电针干预三组(足三里组、内关组、太冲组)大鼠粪便含水率与结肠蠕动波均有所减少,血清中致炎因子IL-1β和5-HT均有所增加、血清中抑炎因子IL-10和SIgA均有所减少,结肠超微结构改善,差异具有统计学意义(P<0.05);其中足三里组此改变最为显著,与内关组和太冲组比较差异有统计学意义(P<0.05);太冲组与内关组比较,差异无统计学意义(P>0.05)。在修复结肠超微结构上,电针干预三组差异无统计学意义。结论电针预处理IBS-D大鼠"足三里""内关""太冲"穴均可降低大鼠粪便含水量,减少肠道蠕动频率,减轻炎症,修复肠道超微结构,增强免疫力,其中足三里穴效果最佳。
Objective Objective To investigate the inflammatory factors IL-1β,IL-10 and 5-HT,SIgA and other related substances in rats with diarrhea irritable bowel syndrome after electroacupuncture at“Zusanli(ST36)”,“Neiguan(PC6)”and“Taichong(LR3)”respectively.Methods We purchased pregnant female Wistar rats and cleaned them into five groups randomly:blank group,model group,“Zusanli(ST36)”group,“Neiguan(PC6)”group and“Taichong(LR3)”group,10 rats in each.A three-factor method(mother-child separation+acetic acid gavage+restraint stress)was used to prepare a diarrhea-type irritable bowel syndrome rat model,and the modeling time was 14 days.The blank group was fed normally without any treatment.The model group was given daily grasping training and mouse clothing putting on and taking off training after modeling.The“Zusanli(ST36)”group was given electroacupuncture at“Zusanli(ST36)”after modeling,and the“Neiguan(PC6)”group was given electroacupuncture at“Neiguan(PC6)”after modeling.After the model of the“Taichong(LR3)”group was built,the electroacupuncture was used to“Taichong(LR3)”.After electroacupuncture intervention,we observed the growth rate of body mass and fecal water content of each group,recorded the number of colonic peristaltic waves of rats in each group within 30 minutes,measured the inflammatory factors IL-1β,IL-10 and 5-HT and SIgA in serum by enzyme-linked immunosorbent assay.The electron microscope was used to observe the changes of colon tissue in each group.Results Compared with those of the blank group,the water content of feces and colon peristaltic waves in the model group were increased,the proinflammatory factors IL-1βand 5-HT in serum were significantly increased,and the anti-inflammatory factors IL-10 and SIgA in serum were significantly reduced.The microstructure was disordered,the microvilli were broken,and the arrangement was uneven.The difference was statistically significant(P<0.05).Compared with those of the model group,the fecal water content and colon peristaltic waves in the three groups[“Zusanli(ST36)”group,“Neiguan(PC6)”group,“Taichong(LR3)”group]were reduced.The proinflammatory factors IL-1βand 5-HT were increased,the serum inflammatory inhibitors IL-10 and SIgA were decreased,and the colon ultrastructure was improved.The difference was statistically significant(P<0.05).This change was most significant in the“Zusanli(ST36)”group,which was significantly different from the“Neiguan(PC6)”group and the“Taichong(LR3)”group(P<0.05).There was no statistically significant difference between the“Taichong(LR3)”group and the“Neiguan(PC6)”group(P>0.05).In repairing the ultrastructure of the colon,there was no statistically significant difference in electroacupuncture intervention among the three groups.Conclusion Electroacupuncture pretreatment of“Zusanli(ST36)”,“Neiguan(PC6)”and“Taichong(LR3)”of diarrhea irritable bowel syndrome rats can reduce the water content of feces in rats,reduce the frequency of intestinal peristalsis,reduce inflammation,repair intestinal ultrastructure and strengthen the immunity.The effect of“Zusanli(ST36)”was the best.
作者
李丹
李佳
吴松
LI Dan;LI Jia;WU Song(Hubei University of Traditional Chinese Medicine/Collaborative Innovation Center of Acupuncture and Moxibustion,Wuhan 430061,Hubei,China)
出处
《辽宁中医杂志》
CAS
2021年第8期238-241,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81973931)
国家自然科学基金青年科学基金(81804165)
作者简介
李丹(1990-),女,湖北黄冈人,硕士研究生,研究方向:针灸效应的基础研究;通讯作者:吴松(1980-),男,湖北武汉人,教授,博士,研究方向:针灸效应的基础研究,E-mail:119065124@qq.com。
