摘要
目的:建立六君子汤化学成分的定性与定量分析方法,明确六君子汤的物质基础。方法:采用超高效液相色谱-四级杆-飞行时间串联质谱法(UPLC-Q-TOF-MS/MS)对六君子汤中的化学成分进行定性分析,利用MassBank、PubChem、ChemSpider、中药系统药理学数据库与分析平台(TCMSP)等数据库并结合相关文献对所得化合物进行鉴定。采用UPLC建立六君子汤中甘草苷、橙皮苷、党参炔苷、甘草素、甘草酸、川陈皮素、桔皮素、白术内酯Ⅱ、白术内酯Ⅰ的定量分析方法并进行分析。结果:结合相关文献、数据库检索和质谱信息,从六君子汤中共鉴定出79个化合物,包括黄酮类31个,萜类15个,含氮化合物14个,苯丙素类6个,有机酸类6个和其他类化合物7个。建立的定量分析方法中9种代表性成分在各自线性范围内线性关系良好,精密度、稳定性、重复性及加样回收率均符合要求,定量结果显示,甘草苷、橙皮苷、党参炔苷、甘草素、甘草酸、川陈皮素、桔皮素、白术内酯Ⅱ、白术内酯Ⅰ在六君子汤中的质量分数分别为0.3765、2.6021、0.0826、0.1281、1.7786、0.0157、0.0067、0.0304、0.0032 mg·g^(-1)。结论:建立的方法可以快速、灵敏、准确地对六君子汤中的化学成分进行分析,明确了六君子汤的物质基础以黄酮类、萜类及含氮化合物为主,并可对其中的9种成分进行定量,可为该方的质量控制、作用机制及临床应用研究奠定基础。
Objective:To establish a qualitative and quantitative analysis method for chemical constituents in Liu Junzitang(LJZT),and to clarify its material basis.Method:The chemical constituents in LJZT were analyzed by ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS),and the resulting compounds were identified by using databases,such as MassBank,PubChem,ChemSpider,Traditional Chinese Medicine Systems Pharmacology Database and Analytical Platform(TCMSP),and by combining with relevant literature.UPLC was used to establish a quantitative method for analysis of 9 compounds in LJZT,including liquiritin,hesperidin,lobetyolin,liquiritigenin,glycyrrhizic acid,nobiletin,tangeretin,atractylenolide Ⅱ and Ⅰ.Result:By combining the relevant literature,database and MS information,a total of 79 compounds were identified from LJZT,including 31 flavonoids,15 terpenoids,14 nitrogen-containing compounds,6 phenylpropanoids,6 organic acids and 7 other compounds.The established quantitative analytical method for the nine representative components showed good linearity within their respective linear ranges,and the precision,stability,reproducibility and recovery were in accordance with the requirements.The quantitative results showed that the contents of liquiritin,hesperidin,lobetyolin,liquiritigenin,glycyrrhizic acid,nobiletin,tangeretin,atractylenolide Ⅱ and Ⅰ in LJZT were 0.3765,2.6021,0.0826,0.1281,1.7786,0.0157,0.0067,0.0304,0.0032 mg·g^(-1),respectively.Conclusion:The established method can quickly,sensitively and accurately analyze the chemical constituents in LJZT,clarify that the material basis of LJZT is mainly flavonoids,terpenoids and nitrogen-containing compounds,and simultaneously determine the contents of the 9 components,which can lay a foundation for the research on quality control,mechanism and clinical application of LJZT.
作者
姜奇瑶
刘臣臣
陈惠玲
黄志峰
赵威
梁颖
潘华峰
卓越
JIANG Qiyao;LIU Chenchen;CHEN Huiling;HUANG Zhifeng;ZHAO Wei;LIANG Ying;PAN Huafeng;ZHUO Yue(Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Vocational and Technical University of Business and Technology,Zhaoqing 526100,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第6期169-178,共10页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省基础与应用基础研究基金-青年基金项目(2021A1515110584)
广州中医药大学中医学学科中医基础理论研究“揭榜挂帅”项目(A1-2606-23-415-453Z07)
国家自然科学基金面上项目(82174319)。
作者简介
第一作者:姜奇瑶,在读硕士,从事中药物质基础研究,E-mail:815553609@qq.com;通信作者:卓越,博士,助理研究员,从事中药药效及毒性物质基础研究,E-mail:zy2008213037@126.com。
