摘要
目的采用超高效液相色谱-电喷雾/四级杆飞行时间串联质谱联用(UPLC-ESI-Q-TOF-MS/MS)技术对双参平肺颗粒中主要化学成分进行准确快速鉴定和归属分类。方法采用SynaptTMQ-TOF型高分辨质谱仪、WatersACQUITYUPLC系统、Waters ACQUITY UPLC?BEH C18色谱柱(100 mm32.1 mm,1.7μm),以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱,采用电喷雾(ESI)离子源,在正、负离子模式下采集数据,通过与对照品的保留时间及质谱数据信息对比,并结合文献报道及质谱数据库中相同成分或同类成分的精确相对分子质量、质谱裂解规律进行匹配,对双参平肺颗粒中复杂成分进行快速准确识别,并明确各成分的药材归属。结果共鉴定了63个化合物,其中萜类化合物24个、酚酸类7个、丹参酮类成分6个、黄酮类14个、其他类12个。结论利用建立的定性分析方法快速准确地识别双参平肺颗粒中主要化学成分,为其进一步的体内显效成分分析、药效物质基础及质量控制研究奠定了基础。
Objective A novel ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS/MS) was applied to establish a method to recognize and classify the main chemical constituents of Shuangshen Pingfei Granules accurately and rapidly. Methods ACQUITY UPLC? BEH C18 chromatographic column(100 mm × 2.1 mm, 1.7 μm) was employed to UPLC analysis with the mobile phase consisting of acetonitrile-0.1% formic acid aqueous solution. ESI ion source was used to ensure the data collected in positive and negative ion mode. The chemical components of Shuangshen Pingfei Granules were identified by comparing with the retention time and the mass data of the reference substance, and consulting literature reports and mass spectrometry database. Results A total of 63 chemical components were identified, including 24 terpenoids, seven phenolic acids, six tanshinones, 14 flavonoids and 12 other classes. Conclusion The qualitative method established in this study could be used to rapidly and accurately identify the main chemical constituents of Shuangshen Pingfei Granules, and lay a foundation for the further analysis of effective ingredients in vivo, pharmacodynamic material basis and quality control research.
作者
陈叶青
范欣生
朱振华
彭国平
段金廒
CHEN Ye-qing;FAN Xin-sheng;ZHU Zhen-hua;PENG Guo-ping;DUAN Jin-ao(College of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第2期321-329,共9页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金青年项目(81904084)
江苏省高等学校自然科学研究-面上项目(18KJB360004)
江苏省中药资源产业化过程协同创新中心重点项目(ZDXM-4).
作者简介
陈叶青(1988—),讲师,博士,从事中药及方剂功效物质基础研究。Tel/Fax:(025)85811945,E-mail:chenyq8804@163.com;通信作者:范欣生,Tel/Fax:(025)85811945,E-mail:fanxsh126@126.com
