摘要
应用超高效液相色谱-四极杆飞行时间质谱联用技术(UHPLC-Q-TOF-MS/MS)对荆芥穗中的化学成分进行定性分析。采用Agilent poroshell 120 SB-C_(18)色谱柱(3.0 mm×100 mm,2.7μm),以0.1%甲酸水(A)-乙腈(B)溶液为流动相进行梯度洗脱,流速0.4 mL·min^(-1),柱温45℃,电喷雾离子源,在正、负离子模式下扫描采集数据,通过对照品比对及PeakView软件分析鉴定化合物。从荆芥穗中共鉴定出97个化合物,其中黄酮类化合物28个,酚酸类化合物23个,脂肪酸类化合物23个,萜类化合物15个以及其他类化合物8个。该研究建立的UHPLC-Q-TOF-MS/MS能快速、准确、较全面地鉴定荆芥穗药材的化学成分,为荆芥穗药效物质基础研究提供参考依据。
The chemical constituents of Schizonepetae Spica were qualitatively analyzed by UHPLC-Q-TOF-MS/MS.An Agilent poroshell 120 SB-C_(18) column(3.0 mm×100 mm,2.7 μm) was used for gradient elution with 0.1% formic acid water(A)-acetonitrile(B) solution as mobile phase at the flow rate of 0.4 mL·min~(-1) and column temperature of 45 ℃.The data were collected by scanning in positive and negative ion modes,and the compounds were identified by comparison of reference materials and PeakView software.Ninety-seven compounds were identified from Schizonepetae Spica,including 28 flavonoids,23 phenolic acids,23 fatty acids,15 terpenoids,and 8 other compounds.The UHPLC-Q-TOF-MS/MS method established in this study can identify the chemical components of Schizonepetae Spica rapidly,accurately,and comprehensively,and provide a basis for the basic study of pharmacodynamic substances of Schizonepetae Spica.
作者
赵毅萌
张礼欣
杨舒昀
王泽琨
李长印
束雅春
ZHAO Yi-meng;ZHANG Li-xin;YANG Shu-yun;WANG Ze-kun;LI Chang-yin;SHU Ya-chun(Department of Pharmacy,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Department of Clinical Pharmacology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Traditional Chinese Medicine Processing Technology Inheritance Base,Jiangsu Provincial Hospital of Chinese Medicine,Nanjing 210029,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第2期420-430,共11页
China Journal of Chinese Materia Medica
基金
国家中医药管理局中药炮制技术传承创新项目(GZY-KJS-2022-053)。
作者简介
通信作者:束雅春,主任中药师,硕士生导师,主要从事中药炮制及中药健康产品研发,E-mail:guzheng0512@163.com;赵毅萌,硕士研究生,E-mail:907054440@qq.com。
