摘要
为了探究不同浓度外源褪黑素对冷驯化后低温胁迫下马铃薯幼苗生长的影响,以云南主栽马铃薯品种合作88为材料,采用人工模拟低温胁迫(4℃14 d,-2℃12 h)的方法,研究了不同外源褪黑素浓度(50,100,150μmol/L)对低温胁迫下马铃薯幼苗生长及活性氧代谢系统的影响,以探讨外源MT缓解低温胁迫对马铃薯幼苗伤害的可行性。结果表明,与低温胁迫的马铃薯幼苗相比:50μmol/L MT处理的幼苗的存活率提高了38.89百分点,株高和茎粗分别提高了2.92%,1.86%,SS、SP和Pro的含量分别增加了12.66%,13.80%,1.96%,POD和CAT的活性分别提高了29.24%,351.62%;100μmol/L MT处理的幼苗的株高和茎粗分别提高了16.97%,2.82%,MDA和H_(2)O_(2)的含量分别降低了19.35%,0.48%,POD和CAT的活性分别提高了55.44%,213.56%,GSH的含量提高了13.61%;150μmol/L MT处理的幼苗的存活率提高了27.78百分点,株高和茎粗分别提高了5.62%,13.20%,MDA的含量降低了9.45%,Pro的含量增加了38.70%,SOD、POD和CAT的活性分别提高了52.61%,25.25%,300.94%,AsA和GSH的含量分别提高了3.48%,3.97%。表明施加外源MT后可以不同程度的通过促进幼苗的生长、降低叶片的MDA和H_(2)O_(2)含量、促进SS、SP、Pro的积累、提高SOD、POD、CAT的活性、增加AsA和GSH的含量,从而缓解低温胁迫对马铃薯幼苗的伤害,进而提高马铃薯幼苗的抗低温胁迫的能力。综上,50μmol/L MT处理可较优得提高马铃薯幼苗在低温胁迫下的抗逆性。
In order to explore the effects of different concentrations of exogenous melatonin on the growth of potato seedlings under low temperature stress after cold acclimation,using Yunnan main potato variety Hezuo 88 as material,the effects of different concentrations of exogenous melatonin(50,100,150μmol/L)on the growth and active oxygen metabolism system of potato seedlings under low temperature stress(4℃14 d,-2℃12 h)were studied.The purpose of this study was to explore the feasibility of exogenous MT in alleviating the injury of potato seedlings caused by low temperature stress.The results showed that compared with potato seedlings under low temperature stress,the survival rate of seedlings treated with 50μmol/L MT increased by 38.89 percentage point,and the plant height and stem diameter increased by 2.92%and 1.86%,the contents of SS,SP and Pro increased by 12.66%,13.80%and 1.96%,the activities of POD and CAT increased by 29.24%and 351.62%.The plant height and stem diameter of seedlings treated with 100μmol/L MT increased by 16.97%and 2.82%,the contents of MDA and H_(2)O_(2) decreased by 19.35%and 0.48%,the activities of POD and CAT increased by 55.44%and 213.56%.The survival rate of seedlings treated with 150μmol/L MT increased by 27.78 percentage point,the plant height and stem diameter increased by 5.62%and 13.20%,the content of MDA decreased by 9.45%,the content of Pro increased by 38.70%,SOD,POD and CAT activity increased by 52.61%,25.25%and 300.94%,and the content of AsA and GSH increased by 3.48%and 3.97%.The results showed that exogenous MT could promote the growth of potato seedlings,reduce the content of MDA and H_(2)O_(2) in leaves,promote the accumulation of SS,SP and Pro,increase the activities of SOD,POD and CAT,and increase the content of AsA and GSH.Thus,it can alleviate the damage of low temperature stress to potato seedlings,and then improve the ability of potato seedlings to resist low temperature stress.In summary,50μmol/L MT treatment could better improve the stress resistance of potato seedlings under low temperature stress.
作者
和秋兰
张航
王正维
万丽嫱
海梅荣
HE Qiulan;ZHANG Hang;WANG Zhengwei;WAN Liqiang;HAI Meirong(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;National-Local Joint Engineering Research Center on Germplasm Innovation&Utilization of Chinese Medicinal Materials in Southwestern China,Kunming 650201,China)
出处
《华北农学报》
CSCD
北大核心
2022年第1期103-111,共9页
Acta Agriculturae Boreali-Sinica
基金
科研发展基金项目(KX900078000)。
关键词
马铃薯
低温胁迫
外源褪黑素
抗氧化系统
Potato
Low temperature stress
Exogenous melatonin
Antioxidant system
作者简介
和秋兰(1997-),女,云南丽江人,在读硕士,主要从事作物生理生态与产量品质形成研究;通讯作者:海梅荣(1973-),女,内蒙古自治区奈曼旗人,教授,博士,硕士生导师,主要从事作物生产生理研究。
