摘要
背景:基于透明质酸的可修饰特性,如何利用各种修饰方式得到满足组织工程软骨修复要求性能的支架已成为目前研究的重点方向。目的:综述经各种改性方式修饰后的透明质酸软骨修复支架的特点及其在软骨组织工程中的优势和应用策略。方法:利用计算机检索中国知网、万方数据、维普和PubMed数据库中有关透明质酸软骨修复支架的文献,检索时间段为2011年1月至2021年3月。中文检索词:"透明质酸、软骨、支架、组织工程",英文检索词:"Hyaluronic acid,Cartilage,scaffold,Tissue engineering",最终纳入72篇文献进行综述。结果与结论:(1)透明质酸的可修饰性为软骨的高效和高质量修复提供了重要应用策略,目前众多相关研究已取得了显著成果:羧基改性后的透明质酸基支架可提高种子细胞的存活率和代谢活性,有良好的生物相容性;羟基改性后的透明质酸支架拥有良好的机械性能和生物降解性;透明质酸的乙酰氨基改性因去乙酰化试剂具有毒性而未用于软骨修复;接枝改性后的透明质酸支架兼具有软骨和软骨下骨的修复功能,可上调软骨基因表达;复合改性后的透明质酸支架具有优异的空间结构,是可调控种子细胞的定向分化并抑制肥大的多功能支架。(2)然而透明质酸基支架在软骨修复中仍面临着一些客观问题和不同的挑战,如目前无法完全实现透明软骨的再生,研究大多处于动物实验阶段,真正应用于临床的研究数量相当有限。(3)近年来,软骨组织工程支架的设计策略已向兼并软骨和软骨下骨修复的方向转变,基于透明质酸的多功能支架将会是未来实现软骨高质量修复的关键。
BACKGROUND:Due to its easy modification characteristics,how to use various modification methods to obtain a scaffold that meets the requirements of tissue engineering cartilage repair has become the focus of current research.OBJECTIVE:To review characteristics of hyaluronic acid cartilage repair scaffolds modified by various modification methods and their advantages and applications in cartilage tissue engineering.METHODS:A computer search of the articles concerning hyaluronic acid cartilage repair scaffolds on CNKI,Wanfang,VIP,and PubMed databases was conducted from January 2011 to March 2021.The"hyaluronic acid,cartilage,scaffold,tissue engineering"in Chinese and English were used as search terms.Finally,72 articles were included for analysis.RESULTS AND CONCLUSION:(1)The modifiability of hyaluronic acid provides an important application strategy for efficient and high-quality cartilage repair.At present,many related researches have achieved remarkable results.Carboxyl modified hyaluronic acid scaffolds can improve the survival rate and metabolic activity of seed cells,and have good biocompatibility.Hydroxy modified hyaluronic acid scaffold has good mechanical properties and biodegradability.The acetamino modification of hyaluronic acid was not used for cartilage repair because of the toxicity of the deacetylation reagent.The grafted hyaluronic acid scaffold can repair cartilage and subchondral bone,and up regulate cartilage gene expression.The composite modified hyaluronic acid scaffold has excellent spatial structure,which can regulate the directional differentiation of seed cells and inhibit hypertrophy,and it is a multifunctional scaffold.(2)However,hyaluronic acid based scaffolds still face some objective problems and different challenges in cartilage repair,for example,hyaluronic acid based scaffolds cannot completely regenerate hyaluronic cartilage at present.Most of the researches are in the animal experimental stage,and the number of clinical researches is quite limited.(3)In recent years,the design strategy of cartilage tissue engineering scaffolds has changed to the direction of annexing cartilage and subchondral bone repair.The multifunctional scaffold based on hyaluronic acid will be the key to achieve high-quality cartilage repair in the future.
作者
刘晖
刘爱峰
张宇
张超
Liu Hui;Liu Aifeng;Zhang Yu;Zhang Chao(Department of Orthopedics and Traumatology,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Regional TCM Bone Injury Diagnosis and Treatment Center of State Administration of Traditional Chinese Medicine,Tianjin 300381,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第34期5518-5524,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81873316),项目负责人:刘爱峰。
关键词
透明质酸
支架
官能团
改性
软骨修复
组织工程
应用策略
综述
hyaluronic acid
scaffolds
functional group
modification
cartilage repair
tissue engineering
application strategy
review
作者简介
第一作者:刘晖,男,1995年生,甘肃省天水市人,汉族,天津中医药大学在读硕士,主要从事膝关节骨性关节炎的治疗和软骨修复研究,https://orcid.org/0000-0003-0481-9796;通讯作者:刘爱峰,男,主任医师,天津中医药大学第一附属医院骨伤科病区主任,天津中医药大学第一附属医院骨伤科,国家中医药管理局区域中医骨伤科诊疗中心,天津市300381, https://orcid.org/0000-0001-7318-1277。
