摘要
背景:细胞膜片技术在组织再生和修复方面具有广阔的应用前景,其在骨组织工程研究领域也具有重要的作用和应用价值。目的:综述细胞膜片技术在骨组织工程中的应用及存在的问题。方法:应用计算机检索CNKI数据库、PubMed数据库及Elsevier数据库中发表的细胞膜片在骨组织工程中应用的相关文献,中文检索词为"细胞膜片技术,细胞膜片,骨,骨缺损,骨修复,组织工程,骨组织工程",英文检索词为"Cell sheet technology,Cell sheet,Bone,Bone defect,Bone repair,Tissue engineering,Bone tissue engineering"。选择2000年1月至2021年7月收录的细胞膜片技术在骨组织工程中应用的相关文章,包括综述、基础及临床研究。结果与结论:细胞膜片技术是一种新型的获取种子细胞的技术,其既能获取想要的种子细胞,同时还能保留丰富的细胞外基质和细胞与细胞间的连接,因此,在组织再生和修复方面具有广阔的应用前景。目前制备细胞膜片的技术主要包括:温度反应系统、机械系统、磁反应系统、光反应系统、pH系统,各种技术在制备细胞膜片的应用方面都具有各自的优缺点。其中,温度反应系统和磁反应系统是目前应用较为广泛的。细胞膜片的种类较多,可根据不同细胞来源分为不同类型的细胞膜片;也可以根据膜片的层数分为单层细胞膜片、双层细胞膜片、多层细胞膜片等。在骨组织工程方面,细胞膜片技术可以单独应用于骨缺损的修复,同时还可以与其他各种支架材料复合从而增加其机械强度并用于骨缺损的修复。此外,在细胞膜片中加入生长因子或者小分子物质为细胞膜片的应用提供了新的思路。
BACKGROUND:Cell sheet technology has broad application prospects in tissue regeneration and repair.What is more,it also has an important role and application value in the field of bone tissue engineering.OBJECTIVE:To review the application and existing problems of cell sheet technology in bone tissue engineering.METHODS:CNKI,PubMed,and Elsevier were searched for relevant articles regarding the application of cell sheet technology in bone tissue engineering published from January 2000 to July 2021.The key words were"cell sheet technology,cell sheet,bone,bone defect,bone repair,tissue engineering,bone tissue engineering"in Chinese and English,respectively.The article types were review,basic and clinical studies.RESULTS AND CONCLUSION:Cell sheet technology is a new technology for obtaining seed cells,which not only acquires the desired seed cells,but also retains the abundant extracellular matrices and cell-cell connections.Therefore,it has broad application prospects in tissue regeneration and repair.The current technologies for cell sheet preparation mainly include:temperature response systems,mechanical systems,magnetic response systems,light response systems,and pH systems.Various technologies have their own advantages and disadvantages in the preparation of cell sheets.Among them,temperature response systems and magnetic response systems are currently widely used.There are many types of cell sheets,which can be divided into different types of cell sheets according to different cell sources.It can also be divided into single-layer cell sheet,double-layer cell sheet,and multi-layer cell sheet according to the number of sheet layers.In terms of bone tissue engineering,the cell sheet technology can be used alone or combined with various other scaffolds to enhance its mechanical strength in the repair of bone defects.In addition,adding growth factors or small molecular substances into cell sheets provides new ideas for the application of cell sheets.
作者
谭先昱
廖文波
Tan Xianyu;Liao Wenbo(Department of Orthopedics,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第12期1944-1949,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(82060415),项目负责人:廖文波。
关键词
细胞膜片技术
组织工程
骨组织工程
支架材料
种子细胞
骨缺损
骨修复
骨
cell sheet technology
tissue engineering
bone tissue engineering
scaffold material
seed cell
bone defect
bone repair
bone
作者简介
第一作者:谭先昱,男,1994年生,贵州省遵义市人,2020年遵义医科大学毕业,硕士,医师,主要从事3D打印个体化骨组织修复材料及脊柱内镜的微创治疗技术研究,https://orcid.org/0000-0002-1269-2708;通讯作者:廖文波,博士,教授,博士生导师,遵义医科大学第二附属医院,贵州省遵义市563000。
