摘要
目的建立经典名方温经汤标准汤剂HPLC指纹图谱,同时对其中9种成分进行含量测定,为其质量控制提供参考。方法采用Kromasil C18色谱柱(250 mm^34.6 mm,5μm),以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,体积流量0.8 mL/min,采用多波长切换法,检测波长分别为220、280、320、380 nm,柱温25℃,对15批温经汤标准汤剂进样分析,采用中药色谱指纹图谱相似度评价软件(2012年版)并结合聚类分析(CA)、主成分分析(PCA)与偏最小二乘法-判别分析(PLS-DA)对结果进行评价,同时进行含量测定。结果建立了温经汤标准汤剂HPLC指纹图谱,15批样品相似度在0.902~0.992,共标定出18个共有峰,指认出其中9个色谱峰(即2号峰没食子酸、5号峰芍药苷、7号峰甘草苷、8号峰阿魏酸、9号峰芹糖异甘草苷、11号峰异甘草苷、14号峰桂皮醛、15号峰甘草酸铵、16号峰丹皮酚),CA、PCA及PLS-DA将15批样品分为2类。定量分析条件方法学考察结果良好,9个成分加样回收率在94.91%~108.16%;15批样品中没食子酸、芍药苷、甘草苷、阿魏酸、芹糖异甘草苷、异甘草苷、桂皮醛、甘草酸铵及丹皮酚的质量分数分别为10.7~31.3、95.8~228.4、18.6~62.4、3.3~8.3、4.8~18.7、2.8~10.6、13.7~108.2、83.9~292.3、31.1~125.5 mg/g。结论建立的HPLC指纹图谱结合多成分同时含量测定的分析方法简便稳定,重复性好,可为经典名方温经汤物质基准的质量评价提供依据。
Objective To establish the HPLC fingerprint and simultaneously determinate nine components of the standard decoction of Wenjing Decoction, so as to provide reference for the quality control of Wenjing Decoction of classical prescriptions. Methods Fingerprints of 15 batches of the standard decoction of Wenjing Decoction were determined by HPLC-PDA, and the control fingerprint was established. All samples were analyzed by Kromasil C18 chromatographic column(250 mm × 4.6 mm, 5 μm) maintained at 25 ℃, and eluted with acetonitrile-0.1% phosphoric acid at the flow rate of 0.8 mL/min, and the detection wavelength was 220, 280, 320 and 380 nm respectively. Combined with cluster analysis(CA), principal component analysis(PCA), and partial least squares discriminant analysis(PLS-DA), the quality of 15 batches of Wenjing Decoction was analyzed. At the same time, the contents of nine active components were determined. Results The similarity of 15 batches of standard decoction of Wenjing Decoction was between 0.902 and 0.992, and a total of 18 common peaks were identified and nine of them(2-gallic acid, 5-paeoniflorin, 7-liquiritin, 8-ferulic acid, 9-isoliquiritin apioside, 11-isoliquiritin, 14-cinnamaldehyde, 15-ammonium glycyrrhetate, 16-paeonol) were quantitative analyzed. CA, PCA and PLS-DA were used to classify the 15 batches of samples into two groups. The results of quantitative analysis were good, and the recovery rate of nine components was 94.91%—108.16%. The content of gallic acid, paeoniflorin, iquiritin, ferulic acid, isoliquiritin apioside, isoliquiritin, cinnamaldehyde, ammonium glycyrrhetate, paeonol in 15 batches of samples were in the range of 10.7—31.3, 95.8—228.4, 18.6—62.4, 3.3—8.3, 4.8—18.7, 2.8—10.6, 13.7—108.2, 83.9—292.3, and 31.1—125.5 mg/g, respectively. Conclusion The HPLC fingerprint combined with the simultaneous determination of multicomponent analysis method eatablished in this experiment are stable and reliable, which can provide the theoretical guidance for the quality evaluation of Wenjing Decoction and its compound preparations.
作者
张越
陈健
李洋
王洪兰
李俊松
狄留庆
ZHANG Yue;CHEN Jian;LI Yang;WANG Hong-lan;LI Jun-song;DI Liu-qing(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Engineering Research Center for Efficient Delivery System of TCM,Nanjing 210023,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第18期4664-4672,共9页
Chinese Traditional and Herbal Drugs
基金
国家中医药管理局中药标准化项目(ZYBZH-C-JS-30)
江苏省科技成果转化专项资金项目(BA2016104)。
关键词
经典名方
温经汤
指纹图谱
含量测定
聚类分析
主成分分析
偏最小二乘法-判别分析
质量评价
桂皮醛
阿魏酸
丹皮酚
芍药苷
甘草苷
甘草酸铵
异甘草苷
芹糖异甘草苷
没食子酸
classical prescriptions
Wenjing Decoction
fingerprint
content determination
cluster analysis
principal component analysis
partial least squares discriminant analysis
quality evaluation
cinnamic aldehyde
ferulic acid
paeonol
paeoniflorin
liquiritin
ammonium glycyrrhetate
isoliquiritin
isoliquiritin apioside
gallic acid
作者简介
张越(1996—),女,在读硕士,研究方向为中药新剂型与新技术。Tel:(025)85811517.E-mail:yuezhang1996@yeah.net;通信作者:王洪兰(1977—),副教授,博士,研究方向为中药及复方药效物质基础。Tel:(025)85811512.E-mail:honglanwang2004@163.com;通信作者:李俊松(1964—),男,博士,教授,研究方向为中药新制剂与新技术。Tel:(025)85811517.E-mail:Lijunsong1964@163.com。
