摘要
目的观察新鲜山药提取物对胃溃疡小鼠溃疡指数、黏膜形态结构,及血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、一氧化氮(NO)表达的影响,探讨其防治胃溃疡的作用机制。方法将100只小鼠随机分为空白对照组、模型组、施维舒组、鲜山药提取物高剂量、鲜山药提取物低剂量共5组;空白对照组和模型组灌胃生理盐水,其余3组灌胃实验药物,灌胃45 d。之后对除空白对照组外的其他4组用消炎痛(2. 5 mg/mL)进行一次性灌胃建立胃溃疡模型,7 h后处死全部小鼠。取血检测血清SOD、GSH-Px活性及NO、MDA蛋白表达;测定溃疡指数,计算溃疡抑制率;取全胃制备胃组织病理切片,HE染色,观察黏膜组织形态改变。结果与模型组比较,鲜山药提取物高剂量组、低剂量组和施维舒组均可减轻消炎痛所致的胃溃疡,胃组织炎细胞浸润及腺体破坏程度较轻;鲜山药提取物高剂量组可降低血清MDA、NO表达,增加SOD、GSH-Px表达含量(均P<0. 05)。结论鲜山药提取物具有预防急性胃溃疡保护胃黏膜的作用,其机制可能与减少MDA、NO含量,增加SOD、GSH-Px表达,发挥抗氧化应激作用有关。
Objective To observe the effect of fresh yam extract on the ulcer index,mucosal morphological structure,expression of serum superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px),malondialdehyde( MDA),and nitric oxide( NO) in mice with gastric ulcers and explore the mechanism of its prevention and treatment of gastric ulcers. Methods We randomly divided 100 mice into blank control,model,teprenone,and high and low dose fresh yam extract groups. Normal saline was administered to blank control and model groups,and experimental drugs were administered to the other three groups for 45 days. Then,the gastric ulcer model was established by one intragastric administration of anti-inflammatory pain( 2. 5 mg/m L) in the groups except the blank control group. All mice were sacrificed after 7 h. The activities of SOD and GSH-Px,and NO and MDA in serum were detected by blood sampling. The ulcer index was measured,and the ulcer inhibition rate was calculated. Pathological sections of gastric tissue were prepared from the whole stomach and stained with HE,and morphological changes of the mucosal tissue were observed. Results Compared with the model group,high dose,low dose,and teprenone groups had reduced gastric ulcers caused by indolence pain,and inflammatory cell infiltration and destruction of the glands in gastric tissue were less. In the high dose group,MDA and NO were decreased in serum,and expression of SOD and GSH-Px was increased( all P<0. 05). Conclusions Fresh yam extract prevents acute gastric ulcers and protects the gastric mucosa,and its mechanism may be related to reducing the serum contents of MDA and NO,and increasing the expression of SOD and GSH-Px for anti-oxidative stress effects.
作者
金佳熹
周冰玉
李柳蓉
冯青秀
柯春娇
覃海音
农雪娟
赵爽
JIN Jiaxi;ZHOU Bingyu;LI Liurong;FENG Qingxiu;KE Chunjiao;QIN Haiyin;NONG Xuejuan;ZHAO Shuang(Youjiang Medical University for Nationalities,Baise 533000,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第3期8-13,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(81760249)
广西自然科学基金项目(2016GXNSFAA380278)
国家级大学生创新创业训练计划项目(201810599002,201710599004)
广西自治区级大学生创新创业训练计划项目(201910599048)
右江民族医学院大学生创新创业训练计划项目(201710599118)
广西研究生教育创新计划项目(JGY2018124)
广西高等教育本科教学改革工程项目(2018JGZ131)
广西职业教育教学改革研究项目(GXGZJG2019B180)。
作者简介
金佳熹(1999-),女,临床医学专业本科生。E-mail:392524722@qq.com;通信作者:赵爽(1980-),女,教授,研究方向:内分泌病理生理学。E-mail:zhsh8000@163.com。
