摘要
以鹰嘴豆为原料,在单因素实验的基础上,利用响应面分析法对超声波辅助提取植物甾醇的工艺条件进行优化,使用柱层析法对提取物进行分离纯化,使用气相色谱质谱联用法(GC-MC)对主要甾醇的种类进行鉴定,并对鹰嘴豆中植物甾醇的抑菌效果进行研究。结果表明,鹰嘴豆植物甾醇的最佳提取工艺为:超声功率450 W,提取时间7 min,液料比10∶1 mL/g,此时提取率为(57.99±3.37) mg/100 g·dw。柱层析法分离纯化植物甾醇的回收率为53.44%。经GC-MS鉴定,分离纯化后鹰嘴豆中植物甾醇以β-谷甾醇(80.43%)、菜油甾醇(12.48%)与豆甾醇(7.09%)为主。抑菌实验结果显示,鹰嘴豆植物甾醇对于大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌以及沙门氏菌均有量效关系。本次研究为进一步开发利用鹰嘴豆中植物甾醇提供一定的参考。
Based on the single factor experiment,chickpea was chosen as raw material and the response surface analysis method was applied to optimize the process conditions of ultrasonic assisted extraction of phytosterols. The column chromatography was used for purification,GC-MS was utilized to analyze the components after purification and its antimicrobial activity was further evaluated. Results showed that,the optimization were as follows:Ultrasonic power 450 W,extraction time 7 min,and the ratio of solvent to material 10∶1 mL/g. Under these conditions,the actual yield was(57.99±3.37) mg/100 g·dw. The recovery rate of phytosterols by column chromatography was 53.44%. After identification of GC-MS,the phytosterols in the chickpeas were mainly β-sitosterol(80.43%),campesterol(12.48%)and stigmasterol(7.09%). The results of bacteriostatic experiments showed that the purified phytosterols had a dose-effect relationship with Escherichia coli,Bacillus subtilis,Staphylococcus aureus and Salmonella. This study provides a reference for further development and utilization of phytosterols in chickpeas.
作者
靳若周
李非凡
曾媛媛
潘赛超
梅晓宏
JIN Ruo-zhou;LI Fei-fan;ZENG yuan-yuan;PAN Sai-chao;MEI Xiao-hong(College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture,Beijing 100083,China;Lab of Storage and Preservation,Quality and Safty&Risk Assessment for Agri-Products(Beijing),Beijing 100083,China)
出处
《食品工业科技》
CAS
北大核心
2019年第24期172-177,184,共7页
Science and Technology of Food Industry
关键词
鹰嘴豆
植物甾醇
响应面法
抑菌活性
chickpea
phytosterol
response surface methodology
antimicrobial activity
作者简介
靳若周(1998-),女,大学本科,研究方向:营养递送系统的研究及应用,E-mail:598703071@qq.com;通讯作者:梅晓宏(1971-),女,博士,副教授,研究方向:营养递送系统的研究及应用,E-mail:mxh@cau.edu.cn。
