摘要
为优化野生火棘叶总甾醇的提取工艺,并探究抗氧化、美白活性。该研究以总甾醇得率为指标,采用单因素实验考察提取溶剂、料液比、超声温度和超声时间对野生火棘叶总甾醇得率的影响,在此基础上利用正交试验设计对提取条件进行优化;采用DPPH、ABTS和亚硝酸盐法,体外评价野生火棘叶总甾醇的抗氧化活性;通过考察对酪氨酸酶抑制作用来评价火棘叶总甾醇的美白活性。结果表明,野生火棘叶总甾醇的最佳提取工艺为提取溶剂无水乙醇,超声温度50℃,料液比1:16 g/mL,超声时间25 min,得到的野生火棘叶总甾醇得率为(11.00±0.03)mg/g;在0.5~5 mg/mL范围内,野生火棘叶总甾醇对DPPH自由基、ABTS+自由基、亚硝酸根离子有很强的清除作用;对酪氨酸酶有很强的抑制作用,且呈现良好的量效关系。该提取工艺稳定、可靠,可用于野生火棘叶总甾醇的提取,野生火棘叶总甾醇可作为一种新型的天然抗氧化剂和酪氨酸酶抑制剂,应用于功能食品中。
The objective of this study was to optimize the extraction process of total sterols from wild Pyracantha fortuneana leaves(WPFL),and explore their antioxidant and whitening activities.The yield of total sterols(WPFLTPS)was used as indicator,and the single factor experiment was used to investigate the effects of extraction solvent,solid-liquid ratio,ultrasonic temperature and ultrasonic time on the yield of WPFLTPS.Based on this,the orthogonal experimental design was used to optimize the extraction conditions.The DPPH(2,2-diphenyl-1-picrylhydrazyl),ABTS[2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]and nitrite methods were used to evaluate the antioxidant activity of WPFLTPS in vitro.The whitening activity was evaluated by the inhibitory effect on tyrosinase.The results showed that for optimal extraction process of WPFLTPS,the extraction solvent was absolute ethanol,ultrasonic temperature was 50℃,solid-liquid ratio was 1:16 g/mL,and ultrasonic time was 25 minutes.Under these conditions,the yield of WPFLTPS was(11.00±0.03)mg/g.In the range of 0.5~5 mg/mL,WPFLTPS had strong scavenging effects on DPPH radicals,ABTS+radicals and nitrite ions,as well as a strong inhibitory effect on tyrosinase,demonstrating a good dose-response relationship.This extraction process was stable and reliable for extracting WPFLTPS.The WPFLTPS can be used as a new,natural antioxidant and tyrosinase inhibitor in functional foods.
作者
刘威良
蔡建
高文荣
朱铃
吕涛
LIU Weiliang;CAI Jian;GAO Wenrong;ZHU Ling;LÜTao(Yunnan Plateau Special Fruit Wine Technology Innovation and Application Engineering Research Center,Qujing Normal University,Qujing 655011,China)
出处
《食品工业科技》
CAS
北大核心
2024年第11期187-194,共8页
Science and Technology of Food Industry
基金
2022年云南省科技厅青年基础研究专项(2106150068Y615)
2023年度云南省地方本科高校基础研究联合专项-面上项目(202301BA070001-080)
云南省地方本科高校基础研究联合专项资金项目-面上项目(202301BA070001-076)
2023年国家级大学生创新创业训练计划项(G202310684011)
曲靖市市校科技创新联合专项资金-重点项目(KJLH2023ZD04,KJLH2023ZD05)
2022年“珠源百人计划”教育人才专项[曲党人才(2022)12号]。
关键词
野生火棘叶
总甾醇
提取工艺
抗氧化
美白
wild Pyracantha fortuneana leaves
total sterols
extraction process
antioxidant
whitening
作者简介
刘威良(1987-),男,博士,讲师,研究方向:植物基功能食品药品研发,E-mail:lwl2046@126.com。;通信作者:吕涛(1984-),男,博士,副教授,研究方向:药食两用植物基抗肿瘤药物研发,E-mail:48295413@qq.com。
