摘要
目的评估松树皮原花青素抑菌作用的稳定性。方法采用K-B纸片扩散法:以松树皮原花青素为材料,以常见腐败细菌为供试菌种,观察松树皮原花青素的抑菌活性以及温度、pH值、紫外线对其抑菌活性的影响。结果松树皮原花青素对革兰阳性菌(金黄色葡萄球菌和枯草芽孢杆菌)有明显的抑制作用,松树皮原花青素对金黄色葡萄球菌和枯草芽孢杆菌的最低抑菌浓度(minimum inhibitory concentration,MIC)分别为12.5mg/mL和6.25mg/mL,在pH值偏中性时对金黄色葡萄球菌和枯草芽孢杆菌有较好的抑制效果,温度及紫外线对其抑菌作用影响较小,而对革兰阴性菌(大肠埃希氏菌及铜绿色假单胞菌)则无明显抑制作用。结论松树皮原花青素对革兰阳性菌有明显的抑制作用,在pH6~pH7范围内对综合杀菌效果较强,温度和紫外线对其抑菌作用几乎没有影响,其抑菌作用受外界影响小,稳定性好。
Objective To study the bacteriostatic activity and stability of pine bark Oligomeric Proantho Cyanidins. Methods By the K-B disk diffusion method, spoilage bacteria as experiment strains, to study the inhibition effects of the pine bark Oligomeric Proantho Cyanidins and the antimicrobial stability under certain temperature, pH, and UV exposure time. Results Pine bark Oligomeric Proantho Cyanidins had significant inhibitory effect on Gram positive bacteria (Staphylococcus aureus and Bacillus Sukatilis), which had better inhibition effects in the media neutral, but temperature and UV had little influence on the antimicrobial effects. The minimum inhibitory concentration (MIC) of pine bark Oligomeric Proantho Cyanidins against Staphylococcus aureus and Bacillus Sukatilis were 12.5 and 6.25 mg/mL, respective. However, on Gram negative bacteria(Escherichia coli and Pseudomonas aeruginosa) there was no obvious inhibition effects. Conclusion The pine bark Oligomeric Proantho Cyanidins has significant inhibitory effect on Gram positive bacteria, the inhibitory effect is stronger in the range of pH 6-7 , and the temperature and UV had a little effect on its antibacterial action, which means that the antibacterial action of pine bark Oligomeric Proantho Cyanidins had good stability.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第2期54-57,60,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
松树皮原花青素
纸片扩散法
抑菌作用
稳定性
革兰阴性菌
革兰阳性菌
pine bark oligomeric proanthocyanidins
disk diffusion method
bacteriostatic activity
stability
gram negative
gram positive
