摘要
目的探讨绞股蓝多糖对MFC胃癌荷瘤小鼠肿瘤生长的抑制及免疫调节作用。方法MFC胃癌细胞接种于BALB/c小鼠右侧腋下,建立MFC胃癌荷瘤小鼠模型。将小鼠随机分为模型组、环磷酰胺组(25mg/kg)及绞股蓝多糖高、低剂量组(100、50mg/kg),每组10只,环磷酰胺组腹腔注射,绞股蓝多糖组灌胃给药,1次/d,给药周期12 d。检测抑瘤率及脏器指数,NK细胞杀伤活性、脾淋巴细胞增殖能力及腹腔巨噬细胞吞噬功能,ELISA检测IFN-γ、IL-2和TNF-α水平,流式细胞仪检测外周血淋巴细胞亚群。结果与模型组比较,各药物处理组小鼠瘤质量均明显降低(P<0.05);环磷酰胺组小鼠体质量明显降低(P<0.05),而绞股蓝多糖组小鼠体质量无明显差异(P>0.05);环磷酰胺组小鼠脾脏指数、胸腺指数、NK细胞杀伤活性、淋巴细胞增殖能力、巨噬细胞吞噬功能、IFN-γ、IL-2、TNF-α水平、CD4+、CD8+细胞水平及CD4+/CD8+比值均明显降低(P<0.05),而绞股蓝多糖各剂量组上述指标均明显增加(P<0.05)。结论绞股蓝多糖对MFC胃癌荷瘤小鼠的肿瘤生长具有抑制作用,该作用与免疫调节作用有关。
AIM To investigate effects of Gynostemma pentaphyllum polysaccharides(GPPs)on tumor growth inhibition and immune regulation in MFC gastric cancer⁃bearing mice.METHODS MFC gastric tumor⁃bearing mice were modeled by inoculation of cultured MFC gastric cancer cells to the right armpit of BALB/c mice.And then the mice were randomly divided into model group,cyclophosphamide group,high and low dose GPPs groups,10 mice in each group.Once a day for a therapeutic period of 12 days,the cyclophosphamide group was intraperito⁃neally injected cyclophosphamide(25 mg/kg),and GPPs groups were given intragastric administration of GPPs(100,50 mg/kg).Subsequently,the mice had their following items checked:tumor inhibition rate and organ in⁃dex,NK cells killing activity,the proliferation ability of splenic lymphocytes and the phagocytic function of abdomi⁃nal macrophages.Their levels of IFN⁃γ,IL⁃2 and TNF⁃αwere determined by ELISA,and their peripheral blood lymphocyte subsets were measured by flow cytometry.RESULTShJCompared with the model group,each other medically intervened group showed significantly reduced tumor mass(P<005);the cyclophosphamide group dis⁃played significantly reduced body mass(P<0.05),while the two GPPs groups revealed no significant difference(P>0.05).The cyclophosphamide group was observed with significantly decreased spleen index,thymus index,NK cell killing activity,lymphocyte proliferation,macrophage phagocytic function,contents of IFN⁃γ,IL⁃2 and TNF⁃α,levels of CD4+and CD8+cells and CD4+/CD8+ratio(P<0.05),in contrast to the two GPPs groups’significantly increased levels of these indicators(P<0.05).CONCLUSION As an immunomodulator,GPPs has an inhibitory effect on tumor growth of MFC gastric cancer bearing mice.
作者
刘艳菊
刘景超
王永飞
LIU Yan⁃ju;LIU Jing⁃chao∗;WANG Yong⁃fei(Medical College,Zhengzhou University of Industrial Technology,Xinzheng 451100,China;The 7th Department of Internal Medicine(The Department of Gastroenterology),People’s Hospital of Xinzheng,Xinzheng 451100,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第12期2876-2881,共6页
Chinese Traditional Patent Medicine
关键词
绞股蓝多糖
MFC胃癌
细胞因子
免疫
Gynostemma pentaphyllum polysaccharides(GPPs)
MFC gastric cancer
cytokines
immunity
作者简介
刘艳菊(1983),女,硕士,主治医师,讲师,从事消化系统疾病的中医药康复研究。Tel:(0371)62623433,18703629166,E mail:3301261614@qq.com;通信作者:刘景超(1954—),女,硕士,教授,从事脾胃疾病的中医药研究。Tel:(0371)62623433,13526802951,E⁃mail:1669224803@qq.com。
