摘要
目的:通过16S rRNA高通量测序技术对比麸炒前后白术水煎液及多糖对脾虚泄泻大鼠肠道菌群的影响,从而为阐明白术麸炒的炮制机理提供理论依据。方法:将SD雄性大鼠30只随机分成正常对照组5只和造模组25只,采用生大黄水煎液致泻结合饮食失节法复制脾虚泄泻模型。造模成功大鼠随机分为模型对照组、生白术水煎液3.6 g/kg组、麸白术水煎液3.6 g/kg组、生白术多糖0.9 g/kg组、麸白术多糖1.0 g/kg组,每组5只。各组灌胃给予相应药物或生理盐水,观察各组大鼠的一般情况,试验结束后取大鼠粪便提取DNA进行高通量16S rRNA测序分析。结果:造模结束后,造模大鼠出现便溏泄泻、体质量增长变缓或体质量呈下降趋势等症状;给药后,与模型对照组相比,各治疗组大鼠体质量增长变快,粪便评分均有所下降,其中生白术多糖0.9 g/kg、麸白术多糖1.0 g/kg组明显下降(P<0.05)。与正常对照组相比,模型对照组大鼠肠道菌群多样性降低,在门、属分类水平上存在菌群结构失衡。与模型对照组相比,各给药组大鼠肠道微生物群落的丰富度和均匀度增加,其中麸白术水煎液3.6 g/kg、麸白术多糖1.0 g/kg多样性明显升高(P<0.05),尤其以麸白术多糖1.0 g/kg的调节作用更优;在门水平上麸白术水煎液3.6 g/kg的Firmicutes菌门水平明显回调(P<0.05);在属水平中,与正常对照组比较,模型对照组Allobaculum、Prevotellaceae_UCG-003属明显下降(P<0.05)。给药干预后,生白术水煎液组3.6 g/kg中Allobaculum属丰度明显升高(P<0.05);麸白术水煎液组3.6 g/kg中Prevotellaceae_UCG-003属丰度明显升高(P<0.05)。结论:白术水煎液及多糖具有缓解腹泻的作用,能够调节脾虚泄泻型大鼠的肠道菌群,上调有益菌丰度,降低有害菌丰度,且对于脾虚泄泻型大鼠肠道菌群多样性的调节作用麸炒作用强于生品。
Objective:Through high-throughput sequencing of 16 S rRNA,the effect of water decoction and polysaccharides of Baizhu(白术)and bran-fried Baizhu on the intestinal flora of rats with Pixuxiexie(脾虚泄泻)syndrome was compared,which laid a theoretical basis for the processing mechanism of bran-fried Baizhu.Methods:Thirty SD male rats were randomly divided into a normal control group(5 rats)and a model group(25 rats).Raw Dahuang(大黄)water decoction was combined with dietary irregularity to induce Pixuxiexie syndrome.The rats in the model group were randomized into model control group,3.6 g/kg Baizhu decoction group,3.6 g/kg bran-fried Baizhu decoction group,0.9 g/kg Baizhu polysaccharide group,and 1.0 g/kg bran-fried Baizhu polysaccharide group,5 in each group.Then,rats were administrated with corresponding drugs or normal saline,and the general conditions of rats in each group were observed.After the experiment,rat feces were collected to extract DNA for high-throughput sequencing of 16 S rRNA.Results:After modeling,rats showed symptoms such as diarrhea,and slow weight gain,or reduced body weight.After administration,compared with the model control group,the administration groups showed fast body weight growth and decrease in stool score,and particularly the decrease in 0.9 g/kg Baizhu polysaccharide group and 1.0 g/kg bran-fried Baizhu polysaccharide group was significant(P<0.05).Compared with the normal control group,the model control group exhibited decrease in intestinal flora diversity and imbalance of the flora structure at the levels of phylum and genus.Compared with the model control group,the administration groups demonstrated growth of the abundance and evenness of the intestinal microbial community,and the increase in diversity in 3.6 g/kg bran-fried Baizhu decoction group and 1.0 g/kg bran-fried Baizhu polysaccharide group was significant(P<0.05),particularly the 1.0 g/kg bran-fried Baizhu polysaccharide group.At the phylum level,3.6 g/kg bran-fried Baizhu decoction up-regulated Firmicutes(P<0.05).At the genus level,the abundance of Allobaculum and Prevotellaceae_UCG-003 was decreased in the model control group compared with that in the normal control group(P<0.05).The abundance of Allobaculum was raised in 3.6 g/kg Baizhu decoction group(P<0.05),and that of Prevotellaceae_UCG-003 was elevated in the bran-fried Baizhu decoction group(P<0.05).Conclusion:The water decoction and polysaccharides of Baizhu have the effect of relieving diarrhea.They regulate the intestinal flora of rats with Pixuxiexie syndrome by increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria.The bran-fried Baizhu shows better effect than the raw medicinal on the intestinal flora of Pixuxiexie syndrome rats.
作者
何晗
但林蔚
王巧珍
陈林
胡媛
王福
刘友平
陈鸿平
He Han;Dan Linwei;Wang Qiaozhen;Chen Lin;Hu Yuan;Wang Fu;Liu Youping;Chen Hongping(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075;College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2022年第2期136-141,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金青年科学基金项目(编号:81303226)
成都中医药大学杏林学者学科人才科研提升计划(编号:XSGG2018007)
关键词
白术
麸炒白术
多糖
肠道菌群
脾虚泄泻
Baizhu(白术)
Bran-fried Baizhu
Polysaccharides
Intestinal flora
Pixuxiexie(脾虚泄泻)syndrome
作者简介
通信作者:陈鸿平,博士,教授,从事中药物质基础与质量标准化研究,E-mail:chen-hongping@126.com;何晗,在读硕士,从事中药物质基础与质量标准化研究,E-mail:907246910@qq.com。
