摘要
目的对比研究不同生长环境和生长年限肉桂出油率、挥发油成分和抑菌活性。方法采用水蒸气蒸馏法提取肉桂挥发油,经气相色谱-质谱技术分离和鉴定不同生长环境和生长年限肉桂油,用归一化法测定其相对百分含量,通过滤纸片法、微量稀释法分别测定抑菌圈直径及最小抑菌浓度(MIC),比较7份肉桂油对常见的3种致病菌的抑菌活性。结果 10年生肉桂出油率最高,为6.41%;20年生肉桂出油率最低,为1.75%,15年生肉桂中疏植出油率最高,为4.48%;7份肉桂油共鉴定出44种化合物,其中相对含量≥ 1%的共有成分为反式肉桂醛(16.23%~73.08%)、α-古巴烯(7.39%~41.70%)、β-杜松烯(2.22%~12.30%)、α-依兰油烯(1.00%~9.16%)。抑菌实验显示,不同生长环境和生长年限肉桂油对供试菌株大肠杆菌、金黄色葡萄球菌均有明显的抑制作用,对绿脓杆菌抑制作用较弱,其中15年生疏植、15年生密植的抑菌活性较好。结论肉桂出油率、肉桂挥发油成分和抑菌活性受生长环境和生长年限的影响,肉桂油中除肉桂醛、α-古巴烯外,还含有其他有效抑菌成分。
Objective To investigate the oil yield, components and antimicrobial activity of the essential oil of Cinnamomi Cortex from different growth environments and growth years. Methods Cinnamon oil was extracted by steam distillation, the chemical components were separated and identified by GC-MS, and the relative content of each component was determined by area normalization. The diameter of the inhibition zone and the minimum inhibitory concentration (MIC) were measured using filter paper method and micro dilution method. The antimicrobial effect of seven copies of cinnamon oil on three common pathogens of respiratory tract were evaluated. Results The highest oil yield was 10-year-old cinnamon (6.41%), the lowest was 20-year-old (1.75%), and sparse planting (4.48%) was the best among 15-year-old cinnamon. A total of 44 compounds were identified in the seven samples. The major common components with a relative content of more than 1% were trans-cinnamaldehyde (16.23%-73.08%),α-copaene (7.39%-41.70%),β-cadinene (2.22%-12.30%), and α-muurolene (1.00%-9.16%). The antibacterial experiments showed that essential oil of cinnamon from different growth environments and growth years had obvious inhibitory effect on the tested strains of Staphylococcus aureus and Escherichia coli, but weaker inhibitory effect on Pseudomonas aeruginosa. Among them, volatile oil of 15-year-old sparse planting and close planting cinnamon had better antimicrobial activity. Conclusion Oil yield, essential oils components and antimicrobial activity of cinnamon were affected by growth environments and growth years. Cinnamon oil contains other effective antimicrobial components besides cinnamaldehyde and α-copaene.
作者
张笮晦
钱信怡
黄广智
李石兰
邓家刚
侯小涛
ZHANG Zuo-hui;QIAN Xin-yi;HUANG Guang-zhi;LI Shi-Ian;DENG Jia-gang;HOU Xiao-tao(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Guangxi GengYuan Flavor and Fragrance Co.,Ltd.,Dongxing 538100,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第12期2990-2996,共7页
Chinese Traditional and Herbal Drugs
基金
广西重点研发计划项目(2017AB35155)
广西中药药效研究重点实验室(16-380-29)
关键词
肉桂油
肉桂醛
α-古巴烯
生长环境
生长年限
抑菌活性
cinnamon oil
cinnamaldehyde
α-copaene
growth environment
growth year
antimicrobial activity
作者简介
张笮晦(1979-),女,博士,讲师,研究方向为天然药物的生物化工、农作物废弃物功能成分的开发与研究。Tel:15978195508,E-mail:zhangzuohui1979@126.com;通信作者:邓家刚,广西终身教授。E-mail:dengjg53@126.com;通信作者:侯小涛,教授,博士生导师。E-mail:xthou@126.com.
