摘要
研究白芷精油、黑胡椒精油、茴香精油、肉桂精油、姜精油和艾草精油对产气荚膜梭菌(Clostridium perfringens,C.perfringens)的抑制作用,筛选出对产气荚膜梭菌有抑菌效果的香辛料精油。采用牛津杯法和两倍稀释法分别判定不同香辛料精油对C.perfringens(ATCC13124、CICC22949、C_1和C_2)的抑菌圈大小和最小抑菌浓度(Minimum inhibitory concentration,MIC)。结果显示:肉桂精油对C.perfringens的抑菌圈直径最大,且对产气荚膜梭菌ATCC13124、CICC22949、C_1和C_2的MIC分别为2.75、2.75、5.5、2.75 mg/m L;艾草精油对C.perfringens的MIC分别为11、2.75、11、2.75 mg/m L;黑胡椒精油对C.perfringens的MIC分别为22、11、11、5.5 mg/m L;茴香精油对C.perfringens的MIC分别为11、5.5、11、2.75 mg/m L;姜精油对C.perfringens的MIC分别为88、22、22、22 mg/m L。结论:除白芷精油以外,其他5种香辛料精油对C.perfringens均有不同程度的抑菌作用,其中肉桂精油对C.perfringens的抑菌效果最佳。
The effect of different spices essential oils （angelica essential oil, black-pepper essential oil, anise essential oil, cinnamon essential oil, ginger essential oil and wormwood essential oil）on the growth of Clostridium perfringen （C.perfringens） was studied to select the spices essential oils which had inhibitory effect on Clostridium perfringens.Oxford cup method and two times dilution method were selected to determine the antibacterial circle and minimum inhibitory concentration （ MIC ） for C.perfringens（ Standard strain ATCC13124 and CICC22949 ,isolated strain Cl and C2 ）.The result showed that the diameter of antibacterial circle of cinnamon essential oil was the largest, and the minimum inhibitory concentration （ MIC ） of cinnamon essential oil on C.perfringens ATCC13124, CICC22949, C2 and C2 were 2.75,2.75,5.5 and 2.75 mg/mL, respectively. The minimum inhibitory concentration of wormwood essential oil on C.perfringens ATCC13124,CICC22949,C2 and C2 were 11,2.75, 11 and 2.75 mg/mL, respectively. The minimum inhibitory concentration of black- pepper essential oil on C.peCringens ATCC13124,CICC22949,C1 and C2 were 22,11,11 and 5.5 mg/mL, respectively.The minimum inhibitory concentration of anise essential oil on C.perfringens ATCC 13124, CICC22949, Ci and C2 were 11,5.5,11 and 2.75 mg/mL, respectively. The minimum inhibitory concentration of ginger essential oil on C.perfringens ATCC13124, CICC22949, C1 and C2 were 88,22,22 and 22 mg/mL,respectively.Conclusion：In addition to angelica essential oil, other five spices essential oils had inhibitory effect on C.perfringens, and the inhibition effect of cinnamon essential oil on C.perfringens was the best.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第23期10-13,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31571856)
关键词
香辛料精油
产气荚膜梭菌
抑菌圈
最小抑菌浓度
spices essential oils
Clostridium peringens
antibacterial circle
minimum inhibitory concentration（ MIC ）
作者简介
王小慧（1994-），女，硕士研究生，研究方向：畜产品加工与质量控制，E-mail：15136277292@163．com。;李苗云（1976-），女，博士，教授，研究方向：肉品加工与安全控制，E-mml：limy7476@126．com。
