摘要
目的探讨24 h内制备浓缩血小板后的去白膜血浆制备冷沉淀的可行性。方法将本站2016年5月～2017年2月采用五联袋(CPDA-1)采集贮存于22℃的400mL全血180袋,分成A1、A2、B1、B2、C1、C2六组,每组30袋。A1组于采血后8 h内,A2、B1、B2、C1、C2组于采血后20～24 h内采用白膜法制备浓缩血小板,分离出白膜上层的血浆速冻作为制备冷沉淀的原料血浆。用血凝分析仪检测冷沉淀凝血因子中Ⅷ因子(FⅧ)、纤维蛋白原(FIB)含量,比较采血后不同制备时间(A1组、A2组)、不同速冻方法(B1组、B2组)、不同冷沉淀制备方法(C1组、C2组)对冷沉淀FⅧ、FIB含量和合格率的影响。结果两种保存时间对冷沉淀FⅧ、FIB质量影响的差异无统计学意义(P>0.05);不同速冻机的速冻效果和冷沉淀制备方法对去白膜血浆制备的冷沉淀FⅧ质量的影响较大,两对照组差异有统计学意义(P <0.05);A1、A2、B1、B2、C1、C2六组FIB均合格,但C1、C2组FIB比较差异有统计学意义(P <0.05)。结论采血后22℃、24h内制备浓缩血小板后的去白膜血浆可作为冷沉淀原料血浆,确保冷沉淀FⅧ、FIB质量的关键是血浆中心温度和冷沉淀制备方法的控制。
Objective To investigate the feasibility of the cryoprecipitation using the dewhite plasma within 24h . Method A total of 180 bags of blood (400mL/bag) were collected and stored under 22℃ from May 2016 to February 2017. The blood was divided into six groups (A1,A2,B1,B2,C1,C2),with 30 bags in each. The concentrated platelets were prepared by albuginea method within 8 h after collection in group A1 and within 20 h-24 h in groups A2,B1,B2, C1 and C2,respectively. The upper-albuginea plasma was separated and quickly frozen as the source for cryoprecipitation. The contents of factor VIII (FVIII) and Fibrinogen (FIB) in cryoprecipitation were detected by coagulation analyzer, and the influence of different preparation time (groups A1 and A2),quick-freezing methods (groups B1 and B2) and cryoprecipitation preparation methods (groups C1 and C2) on the contents of FVIII and FIB in cryoprecipitation and the qualified rates were compared. Result The influence of two storage times on the quality of FVIII and FIB in cryoprecipitation was not statistically significant (P >0.05). The freezing by instant freezers and preparation methods of cryoprecipitation gave rise to a remarkable impact on the quality of FVIII(P <0.05). The FIB in groups A1,A2,B1,B2,C1 and C2 was qualified but a statistical difference (P <0.05) was noted FIB between groups C1 and C2. Conclusion The dewhite plasma within 24 h at 22℃ after concentration of platelets might be used as the source for cryoprecipitation,the temperature of the plasma and preparation method of cryoprecipitation play a crucial role in guaranteeing the quality of FⅧ and FIBs.
作者
梁若鹄
黄彦
邓金莲
龚慧英
陈龙菊
符雪丽
李诗敏
LIANG Ruo-hu;HUANG Yan;DENGJin-lian(Department of Component Transfusion ,Maoming Central Blood Station ,Maoming ,Guangdong 525000)
出处
《临床输血与检验》
CAS
2019年第2期134-137,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
广东省茂名市科技计划项目(No.20140336)资助
关键词
去白膜血浆
冷沉淀
Ⅷ因子
纤维蛋白原
质量控制
Buffy-coat-removed plasma
Cryoprecipitation
VIII factor
Fibrinogen
Quality control
作者简介
梁若鹄(1973–),广东茂名人,女,副主任护师,本科,主要从事临床输血工作,Tel,18929768108,E-mail,liangruohu1973103@163.com。
