摘要
目的探究血必净对百草枯中毒大鼠肺组织中Caspase-3表达以及对血浆中炎症因子表达的影响。方法选择50只健康大鼠随机（随机数字法）等分为5组，即正常组（Control），百草枯中毒组（Paraquat），低剂量血必净组（LD），中剂量血必净组（MD），高剂量血必净组（HD），一次性灌胃25mg／kg的百草枯溶液建立动物模型，LD、MD和HD组分别腹腔注射1mL浓度分别为14μmol／kg，28μmol／kg，56μmol／kg的血必净溶液，Control组和Paraquat组注射同等剂量生理盐水，24h后ELISA法检测血浆中TNF-α，IL-6的表达水平，免疫组化法检测右肺组织中Caspase-3的表达水平，TUNEL法检测右肺组织中细胞凋亡情况。结果在百草枯中毒后，大鼠血浆中TNF-α，IL-6水平显著升高，肺组织中Caspase-3蛋白表达显著升高，（P〈0．01），在经过不同剂量血必净治疗后，血浆中TNF-α，IL-6水平、肺组织中Caspase-3蛋白表达较Paraquat组相比显著降低，但仍然高于正常组（P〈0．05），且MD组和HD组肺组织中细胞凋亡水平明显比Paraquat组中低（P〈0．05）。结论血必净能够显著降低百草枯中毒大鼠血浆中炎症因子水平，并抑制肺组织中Caspase-3蛋白的表达，减少细胞凋亡，缓解大鼠急性肺组织损伤。
Objective To study the effect of xuebijing on the expression of Caspase-3 in lung tissues and inflammatory factors in serum in acute respiratory distress syndrome rat model with paraquat poisoning. Methods Selecting 50 healthy rats and dividing into 5 groups randomly, Control group, Paraquat group, Low-dose xuebijing group（LD）, Middle-dose xuebijing group（MD）, High-dose xuebijing group（HD）. Using 25 mg/kg paraquat solution to establish the animal model. 14 μmol/kg, 28 μmol/kg, 56 μmol/kg xuebijing solution were injected into the abdominal cavity in LD, MD and HD group respectively, and the same dose of normal saline was injected into Control and Paraquat group. The expression levels of TNF-α and IL-6 in plasma were detected by ELISA after 24 hours, Immunohistochemistry was used to detect the expression levels of caspase-3 in the right lung tissue, and TUNEL method was used to detect apoptosis in the right lung tissue. Results The expression levels of TNF-α and IL-6 in plasma were significantly increased after paraquat poisoning, and expression of Caspase-3 protein was also significantly increased in lung tissue（P〈0.01）. After different dose of xuebijing treatment, the levels of TNF-α and IL-6 in plasma and the protein expression of Caspase-3 in lung tissue were much lower than those in Paraquat group, but still higher than those in Control group（P〈0.05）, the apoptosis levels in MD and HD group were much lower than those in Paraquat group（P〈0.05）. Conclusions Xuebijing can significantly reduce the level of inflammatory factors in plasma, suppress the Caspase-3 protein expression and apoptosis in lung tissue in acute respiratory distress syndrome rat model with paraquat poisoning.
作者
曹丽萍
温彦芳
曹昌魁
杨志洲
孙兆瑞
聂时南
Cao Liping;Wen Yanfang;Cao Changkui;Yang Zhizhou;Sun Zhaorui;Nie Shinan(Department of Emergency Medicine,Nanjing General Hospital,Zhongshan East Road 305,Nanjing,210002,China)
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2018年第10期1121-1125,共5页
Chinese Journal of Emergency Medicine
基金
国家自然科学基金（81401583,81701894）
江苏省科教强卫工程青年医学人才项目（QNRC2016908,QNRC2016909）
江苏省社会发展面上项目（BE2017720）
作者简介
通信作者：聂时南，Email：shn_nie@sina．com
