摘要
目的研究川芎Ligusticum chuanxiong的化学成分,并探讨从川芎中首次发现人参皂苷的意义。方法采用大孔吸附树脂、Sephadex LH-20、硅胶等柱色谱,制备薄层色谱、半制备高效液相色谱等方法分离、纯化液质联用分析中显示的皂苷类成分,通过理化性质和NMR、MS等波谱学方法鉴定其结构。结果从川芎正丁醇提取物中分离得到了3个人参皂苷类化合物,分别为(20S)-人参皂苷Rh_1(1)、(20R)-人参皂苷Rh1(2)、(20R)-人参皂苷Rg3(3)。结论以上化合物均首次从伞形科藁本属植物川芎中分离得到,该发现对阐明川芎药效物质基础具有重要意义,也为进一步证实人参与川芎在物种进化和传统功效上的关联提供参考资料。
Objective To investigate the chemical constituents of the rhizomes of Ligusticum chuanxiong and discuss the significance of first discovery of ginsenosides from the plant. Methods The compounds were isolated and repeatedly purified by column chromatographies such as macroperous resin, Sephadex LH-20, silica gel, and preparative TLC as well as semi-preparative RP-HPLC. Their structures were elucidated by physicochemical properties, NMR, and MS spectral analyses. Results Three ginsenoside compounds were isolated from the n-butanol extracts of rhizomes of L. chuanxiong, and their structures were identified as（20S）-ginsenoside Rh1（1）,（20R）-ginsenoside Rh1（2）, and（20R）-ginsenoside Rg3（3）. Conclusion Ginsenosides are isolated from the genus Ligusticum（Umbelliferae） for the first time, it is of great significance for clarifying pharmacodynamic material basis of the rhizomes of L. chuanxiong. These results also provide the reference data for further verifying the relevance of plant evolution and traditional efficacy between L. chuanxiong and Panax ginseng.
出处
《中草药》
CAS
CSCD
北大核心
2017年第18期3677-3681,共5页
Chinese Traditional and Herbal Drugs
基金
四川省科技支撑计划(2015SZ0031)
科技基础性工作专项计划(2015FY111500-140)
四川省川产道地药材综合开发与区域发展项目(2017ZY002)
作者简介
蒲忠慧，在读博士，研究方向为中药药效物质基础研究。E-mail：zhonghui．pu@163．com
通信作者 彭成，研究员。Tel：（028）61800018 E-mail：pengchengchengdu@126．com
通信作者 熊亮，研究员。Tel：（028）61800231 E-mail：xiling0505@126．com
