摘要
目的建立超高效液相色谱-质谱联用(UPLC-Q-TOF-MS)法分析芩百清肺浓缩丸(黄芩、百部、紫菀等)中的化学成分。方法分析采用Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm);流动相为0.1%甲酸水(A)-0.1%甲酸乙腈(B),梯度洗脱;体积流量0.3 m L/min;柱温35℃。结果共鉴定出78个化合物,14个来自黄芩,29个来自百部,10个来自麦冬,13个来自紫菀,3个来自桔梗,9个来自地龙。结论该方法准确快速,可用于芩百清肺浓缩丸的鉴定分析。
AIM To establish an ultra performance liquid chromatography quadrupole time of flight mass spectrometry（UPLC-Q-TOF-MS） method for analyzing the chemical constituents in Qinbai Qingfei Concentrated Pills（Scutellariae Radix,Stemonae Radix,Asteris Radix et Rhizoma,etc.）.METHODS The analysis was performed on an Acquity UPLC BEH C18column（2.1 mm × 100 mm,1.7 μm）,mobile phase was 0.1% formic acid aqueous solution（A）-0.1% formic acid and acetonitrile（B） with gradient elution,flow rate was 0.3 m L/min,and column temperature was maintained at 35 ℃.RESULTS Total seventy-eight constituents were identified,fourteen of which came from Scutellariae Radix,twenty-nine of which came from Stemonae Radix,ten of which came from Ophiopogonis Radix,thirteen of which came from Asteris Radix et Rhizoma,three of which came from Platycodonis Radix,nine of which came from Pheretima.CONCLUSION This method is accurate and rapid,which can be used for the identification and analysis of Qinbai Qingfei Concentrated Pills.
出处
《中成药》
CAS
CSCD
北大核心
2016年第6期1303-1310,共8页
Chinese Traditional Patent Medicine
基金
国家"重大新药创制"科技重大专项(2010ZX09101-104)
黑龙江省青年基金(QC2011C114)
作者简介
褚衍涛（1990-），男，硕士生，从事中药新药研发工作。Tel：（0451）55653086-6902，E-mail：chuyantao2010@126．com
通信作者：王伟明（1966-），女，博士，研究员，从事中药新药研发工作。Tel：（0451）55665478，E-mail：zyyjy@163．com
