摘要
目的:观察白附片对健康大鼠及肾阳虚证模型大鼠心电、心肌组织形态结构影响的差异。方法:80只SD雄性大鼠随机分为健康组和肾阳虚证模型组,每组各自用5个梯度浓度的白附片醇提物连续灌胃14d。描记大鼠心电图,记录心率(BPM)、峰-峰值(P-P)及心律失常情况,光镜观察心脏病理形态改变。结果:白附片使健康和模型大鼠的BPM加快、P-P增大及心律失常发生,随着剂量的增加,模型大鼠BPM加快的幅度较小,心律失常发生率较低;白附片使健康和模型大鼠心脏病理形态改变,同一剂量时,模型大鼠的改变程度较轻。结论:白附片对健康和肾阳虚模型大鼠的心脏毒性与剂量相关,肾阳虚模型大鼠的心脏毒性反应较轻。
Object：To investigate the differences of electrocardiogram（ECG） and morphological structure of cardiac tissue disturbed by ethanol extracts of white prepared lateral root of aconite（WPLRA） in healthy rats and kidney deficiency rats.Methods： Eighty male Sprague Dawley rats were divided randomly into healthy group and kidney deficiency model group,with forty rats in each group.Every group rats were respectively divided into four treatment groups at different intragastric administration dosages and blank control group,with eight rats in each group.Treatment group rats were administered ethanol extracts of white prepared lateral root of aconite（WPLRA）,however blank control group corresponding normal saline,every day for two weeks.All rats Ⅱ couplet electrocardiogram were traced,heartbeat rate（BPM） and peak-to-peak（P-P） as well as arrhythmia were recorded.Besides,all rats histopathological changes of cardiac was observed by HE.Results： WPLRA made healthy and model rats＇ heartbeat rate accelerate.Higher dose,faster heartbeat rate.But accelerating extent of model rats was smaller than that of normal rats.WPLRA caused healthy and model rats＇ P-P value increase and both increasing extent was consistent with adding dose.WPLRA also led to arrhythmia and higher dose WPLRA caused more serious arrhythmia and higher occurring rate of arrhythmia.In addition,in same dose,the occurring rate of arrhythmia of model rats was lower than that of healthy rats.WPLRA caused cardiac histopathological changes of both healthy rats and model rats.Cardiac pathological damage became more and more serious gradually with adding dose.And in same dose administration,the cardiac pathological damage of model rats was slighter than that of normal rats.Conclusion： There is the dose-related feature on cardiac toxicity of WPLRA.The cardiac toxicity was relative to physiological status.The same dosage WPLRA could rouse a weaker cardiac toxicity in kidney deficiency rats than that in healthy rats.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2011年第10期1080-1083,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(30825047,30902000)
北京市教育委员会科技研究面上项目(KM200910025012)
中国中医科学院第三批自主选题项目(Z0138)
关键词
白附片
肾阳虚证大鼠模型
心电图
心脏组织病理
white prepared lateral root of aconite（WPLRA）
kidney deficiency rat model
electrocardiogram（ECG）
cardiac histopathological changes
作者简介
谭勇（1976-），男，陕西宝鸡人，副主任医师，医学博士，从事中医病证关联研究和中药毒性评价研究。
李健（1970-），男，河北昌黎人，副教授，医学博士，从事抗炎及免疫调节作用中药及单体化合物的药理机制研究。
通讯作者：吕爱平，男，教授，博士研究生导师，从事中医病证关联研究和中药复方机制研究，E-mail：lap64067611@126．com
