摘要
目的探讨灯盏花素对糖尿病大鼠转化生长因子-β1(TGF-β1)表达和肾功能的影响。方法选健康雄性昆明种SD大鼠70只,随机分为正常对照组(N)20只、糖尿病组(DM)和灯盏花治疗组(EB)各25只,后两组用链脲菌素(streptozotocin,STZ)诱导建立糖尿病肾病大鼠模型,EB组行灯盏花素20mg·kg-1·d-1腹腔内注射,N组和DM组行等量生理盐水腹腔内注射,三组给药后2w和6w宰杀,收集标本检测尿肌酐、血糖、血肌酐、尿素氮和血清TGF-β1,观察各组肾小球平均面积(AC)和体积(VC)的变化。结果①TGF-β1比较:糖尿病组>灯盏花组>正常组;②灯盏花组及糖尿病组与正常组相比体重下降,肾指数、肾小球面积、体积升高,且灯盏花组较糖尿病组体重增加,肾指数、肾小球面积、体积下降(P<0.05);③血尿素氮、肌酐比较:糖尿病组>灯盏花组>正常组,灯盏花组较糖尿病组降低,且肌酐降低以2w组更明显(P<0.01)。结论灯盏花素能够抑制糖尿病大鼠血清TGF-β1的表达和肾脏肥大。
Objective To investigate the effect of Erigeron breviscapine on the expression of transforming growth factor-batel （TGF- β1） and the renal function in diabetic rats. Methods 70 normal male rats were selected. Rats were randomly divided into three groups： normal group （N） , diabetic group （DM） , and breviscapine treatment group （EB） . DM and EB groups were induced by injection streptozotocin （STZ） to become the model of the diabetic nephropathy. Erigeron breviscapine was injected into rats of EB group by the eoeliac cavity. Normal saline was injected into rats of N and DM groups by the same cavity. Rats of three groups were killed after 2weeks and 6weeks of giving medicine, then blood specimen was collected to examine urine creatinine （Ucr） , blood glucose （Glu） , serum creatinine （Cre） , blood urea nitrogen （Bun） and TGF-β1 concentrations, The changes of the glomerulus area （AC） and volume （VC） in each group were observed. Results ①Comparison of TGF-β1：diabetic group 〉 breviscapine treatment group 〉 normal group.②Comparing with normal group, the weight of dia- betic group and breviscapine group decreased, renal index, AC and VC increased. Comparing with diabetic group, the weight of breviscapine group was increased, renal index, AC and volume were decreased （P 〈 0.05） . ③Comparison of Cre and Bun ： diabetic group 〉 breviscapine treatment group 〉 normal group. Comparison with diabetic group, the levels of Cre and Bun of breviscapine group were lower. Cre decrease was more obvious in 2 weeks （P 〈 0.01） . Conchlsion Erigeron breviscapus can repress the expression of serum TGF-β1 and repress kidney overgrowth in diabetic rats.
出处
《云南医药》
CAS
2007年第4期324-327,共4页
Medicine and Pharmacy of Yunnan
基金
云南省教育厅科学基金项目
NO:03Y457C
关键词
灯盏花素
转化生长因子-Β1
大鼠
糖尿病肾病
Erigeron breviscapus
Transforming growth factor-β1
Rats
Diabetes nephropathy.
作者简介
赵燕（1978-）女，在读硕士，研究方向为内分泌及代谢病学。
