摘要
目的以海蜇为原料,研究海洋软体动物的糖胺聚糖的提取、纯化工艺步骤,优化酶解条件;同时,对其糖胺聚糖的降血脂作用进行初探。方法经多酶水解、醇沉后得海蜇糖胺聚糖粗制品(R-GAG),再经吸附、透析、醇沉及CTAB络合沉淀后得海蜇糖胺聚糖纯化物;动物实验,造高脂血症小鼠模型,灌喂海蜇糖胺聚糖粗品20d后,分别通过LRC法、乙酰丙酮显色法测定小鼠血清总胆固醇和甘油三酯水平。结果海蜇糖胺聚糖粗提工艺中最适酶解条件是:木瓜蛋白酶1.5%,枯草蛋白酶1.5%,酶解5h;动物实验,10 g.kg-1R-GAG灌喂高脂血症小鼠20d后,其血清总胆固醇(TC)、甘油三酯(TG)水平分别下降为6.83±1.09(P<0.05),4.06±1.28mmol.L-1(P<0.01)。结论经多酶水解得海蜇糖胺聚糖粗制品具有显著的降血脂作用。
Objective To study the extraction and purification of glycosaminoglycans from Rhopilerna esculenta Kishinouye, optimize the enzymolysis process, and its antihyperlipidemia effects were studied. Methods Extraction of glycosaminoglycans （GAG） from Rhopilerna esculenta Kishinouye by hydrolyzation method with papain, subtilisin and trypsin neutral proteinase, ethanol precipitation. The rude product of glycosaminoglycans （GAG） was purified and fractionated by the methods of adsorption, dialysis, ethanol precipitation and CTAB precipitation. The experimental hyperlipidemia mice induced by high fat forage were studied. The total cholesterol （TC） and triglyceride （TG） levels were determined. Results Optimum enzymolysis process： papain neutral proteinase1. 5%, subtilisin neutral proteinase1. 5%, treated for 5h; The result showed that the levels of TC and TG decreased to 6.83±1.09 mmol · L^-1 （p〈 0.05 ）, 4.06 ± 1. 28mmol · L^-1 （P 〈 0. 01 ） respectively, when the experi- mental hyperlipidemia mice treated with R-GAG at the doses of 10g · kg^-1 for 20d. Conclusion The rude extracts of glycosaminoglycans （GAG） from Rhopilema esculenta by hydrolyzation method possessed significant anti-hyperlipidemia action.
出处
《中国海洋药物》
CAS
CSCD
2007年第4期41-44,共4页
Chinese Journal of Marine Drugs
关键词
海蜇
糖胺聚糖
提取
纯化
降血脂
Rhopilerna esculenta Kishinouye
glycosaminoglycan （GAG）
extraction
purification
anti-hyperlipidemia
作者简介
金晓石，男，硕士研究生 Tel：（0759）2362247；E-mail：kingstoneocean@163.com
