摘要
通过响应面法优化海蜇胶原蛋白的提取工艺,并通过小鼠皮肤创伤模型研究海蜇胶原蛋白的促愈合作用。通过单因素试验选取因素与水平,根据Box-Benhnken中心组合试验设计原理,在单因素基础上采用三因素三水平响应面分析法(RSM),对海蜇胶原蛋白的酸法提取工艺进行优化;采用小鼠皮肤创伤模型,创面外用胶原蛋白,并以云南白药处理为阳性对照,通过十字测量法测量创面大小并计算创面愈合率。结果显示海蜇胶原蛋白的最佳提取工艺条件为提取时间62.64h、液料比23.6:1,醋酸浓度0.562mol/L;动物实验表明海蜇胶原蛋白能够明显缩短伤口愈合时间,对创面修复有促进作用。响应面法优化得到的提取条件准确可靠;海蜇胶原蛋白具有明显的促愈合作用。
The optimum condition of jellyfish （Rhopilema esculentum Kiahinouye 1891） collagen was studied, and the effect of jellyfish collagen on promoting the wound healing was studied based on the model of mouse skin wounds. Experimental factors and their levels were determined by single-factor tests, and then a three factors and three levels test was designed based on the principle of Box-Benhnken design. The extraction conditions were optimized through response surface method （RSM）. In the experiment of wound healing in mouse, cutting-skin injures model in mouse were created and wounds were treated with collagen, at the same time yunnan baiyao was used as positive control. The size of wounds was measured through cross measurement, and the healing rates were calculated. Results showed the optimal conditions of jellyfish collagen extraction were obtained as following： extraction time 62.64 h, the solid/water ratio of 1：23.6, acetic acid concentration of 0.562 mol/L. Animal tests showed that treatment with collagen could significantly accelerate the process of re-epithelialization, and promote wound healing.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期19-23,共5页
Food Science
基金
浙江省自然科学基金项目(Y304358)
宁波市科技局重点资助项目(200120041)
关键词
海蜇
胶原蛋白
响应面
伤口愈合
jellyfish
collagen
Response surface method （RSM）
wound healing
作者简介
丁进锋（1984-），男，硕士研究生，研究方向为水产品加工与贮藏工程。E—mail：djfeng-0617@163．com
通信作者：苏秀榕（1956-），女，教授，博士，研究方向为食品科学与工程、生物化学与分子生物学。E-mail：suxiurong@nbu．exiu．cn
