摘要
探讨珠母贝氨基多糖的分离纯化及其抗肿瘤活性。马氏珠母贝全脏器经胰酶和枯草杆菌蛋白酶双酶水解、醇沉后得到氨基多糖粗品(CPG),再经DEAE-52-纤维素柱纯化,并采用MTT法检测其体外抗肿瘤活性。结果表明:CPG和经DEAE-52-纤维素柱纯化级分GAG-1,GAG-2中总氨基己糖的质量分数分别为45.9%,56.5%和59.2%,级分GAG-1硫酸基的质量分数高达13.8%;体外抗肿瘤试验表明CPG,GAG-1和GAG-2具有抑制肿瘤细胞生长的作用,对HL-60细胞的抑制率分别为54.6%,50.0%和35.4%,且能显著增敏化疗药物5-Fu抑制肿瘤细胞的作用,其中GAG-1为主要的活性成分。
Crude glycosaminoglycan(CPG) from the whole viscera of Pinctada martensii was isolated with the two-enzyme hydrolysis and purified by the chromatograph.And the antitumor activity was studied with MTT colormetric assay. The CPG could be separated into two fractions(GAG-1, GAG-2) by the DEAE-52-cellulose ion exchange chromatograph. The contents of hexosamine of CPG,GAG-1, GAG-2 were 54.6%,50.0%,35.4%,respectively. The sulfate of GAG-2 was 13.8%. The inhibiting rates of CPG, GAG-1 and GAG-2 to HL-60 cells were 54.6%, 50.0% and 35.4%,respectively. The inhibition to HL-60 of 5-Fu could be increased by combined use with CPG, GAG-1 and GAG-2, GAG-1 was the main active fraction.
出处
《中国海洋药物》
CAS
CSCD
2005年第2期32-36,共5页
Chinese Journal of Marine Drugs
关键词
珠母贝
氨基多糖
柱层析
抗肿瘤活性
Pinctada martensis
glycosaminoglycan(GAG)
chromatography purification method
antitumor activity
